Folkesson H G, Weström B R, Lundin S, Karlsson B W
Department of Zoophysiology, University of Lund, Sweden.
Acta Physiol Scand. 1988 Jul;133(3):283-8. doi: 10.1111/j.1748-1716.1988.tb08409.x.
The nonapeptide 1-deamino-cysteine-8-D-arginine vasopressin (dDAVP) was gavage-fed together with cow's milk whey protein to young, developing rats. The transepithelial passage of dDAVP in the gastrointestinal (GI) tract was assessed by a specific RIA as immunoreactive levels in blood serum extracts and as urinary excretion of dDAVP 0.5-8 h after feeding. In 14-day-old rats the passage of dDAVP was higher than in 30-day-old rats, since the 14-day-old rats had significantly higher serum levels (5-10 times) 0.5-2 h after feeding and a urinary excretion approaching 0.15% of the administered amount after 8 h. In the 30-day-old rats urinary excretion increased up to 0.05% after 2 h and then levelled off. It was also clear that 30-day-old rats had a slower transfer to and faster elimination from serum than 14-day-old rats. dDAVP appeared to be passed over the GI tract mucosa independently of intestinal proteolysis since feeding it to 30-day-old rats together with the proteinase inhibitors, soya-bean trypsin inhibitor and pepstatin did not influence the serum or urinary levels. Thus, dDAVP was taken up from the GI tract into the blood circulation and excreted in the urine of young rats. The decrease in the passage of dDAVP found around weaning appears to be related to developmental processes affecting the permeability of the intestinal epithelium rather than intestinal proteolysis.
将九肽1-去氨基-半胱氨酸-8-D-精氨酸加压素（dDAVP）与牛乳清蛋白一起经口强饲给幼年发育中的大鼠。通过特定的放射免疫分析法，以血清提取物中的免疫反应水平以及喂食后0.5 - 8小时dDAVP的尿排泄量来评估dDAVP在胃肠道（GI）中的跨上皮转运情况。在14日龄的大鼠中，dDAVP的转运高于30日龄的大鼠，因为14日龄的大鼠在喂食后0.5 - 2小时血清水平显著更高（5 - 10倍），且8小时后尿排泄量接近给药量的0.15%。在30日龄的大鼠中，2小时后尿排泄量增加至0.05%，然后趋于平稳。同样明显的是，30日龄的大鼠与14日龄的大鼠相比，向血清的转运较慢且从血清中的清除较快。dDAVP似乎独立于肠道蛋白水解作用穿过胃肠道黏膜，因为将其与蛋白酶抑制剂大豆胰蛋白酶抑制剂和胃蛋白酶抑制剂一起喂给30日龄的大鼠不会影响血清或尿液水平。因此，dDAVP从胃肠道被吸收进入血液循环并在幼鼠尿液中排泄。断奶前后发现的dDAVP转运减少似乎与影响肠上皮通透性的发育过程有关，而非肠道蛋白水解作用。
