São Paulo State University (UNESP), Department of Dental Materials and Prosthodontics, 14801-907, Brazil.
Interdisciplinary Laboratory of Electrochemistry and Ceramics, University Federal of São Carlos, P.O. Box 676, 13565-905 São Carlos, SP, Brazil.
Mater Sci Eng C Mater Biol Appl. 2020 Jun;111:110765. doi: 10.1016/j.msec.2020.110765. Epub 2020 Feb 20.
Crystal morphology with different surfaces is important for improving the antibacterial activity of materials. In this experimental and theoretical study, the antibacterial activity of β-AgMoO microcrystals against the Gram-positive bacteria, namely, methicillin-resistant Staphylococcus aureus (MRSA), and the Gram-negative bacteria, namely, Escherichia coli (E. coli), was investigated. In this study, β-AgMoO crystals with different morphologies were synthetized by a simple co-precipitation method using three different solvents. The antimicrobial efficacy of the obtained microcrystals against both bacteria increased according to the solvent used in the following order: water < ammonia < ethanol. Supported by experimental evidence, a correlation between morphology, surface energy, and antibacterial performance was established. By using the theoretical Wulff construction, which was obtained by means of density functional calculations, the morphologies with large exposition of the (001) surface exhibited superior antibacterial activity. This study provides a low cost route for synthesizing β-AgMoO crystals and a guideline for enhancing the biological effect of biocides on pathogenic bacteria by the morphological modulation.
不同表面形态的晶体对于提高材料的抗菌活性很重要。在这项实验和理论研究中，研究了 β-AgMoO 微晶体对革兰氏阳性菌，即耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性菌，即大肠杆菌（E. coli）的抗菌活性。在这项研究中，通过使用三种不同溶剂的简单共沉淀法合成了具有不同形态的 β-AgMoO 晶体。根据所用溶剂，所得微晶体对两种细菌的抗菌效果依次增加：水<氨水<乙醇。实验证据支持下，建立了形态、表面能和抗菌性能之间的相关性。通过使用密度泛函计算获得的理论 Wulff 构造，（001）表面暴露较多的形态表现出优异的抗菌活性。本研究提供了一种低成本合成 β-AgMoO 晶体的方法，并为通过形态调制增强杀菌剂对致病菌的生物效应提供了指导。
