Ren Zhitao, Lan Qingping, Chen Yan, Chan Yuet Wa Judy, Mahady Gail B, Lee Simon Ming-Yuen
State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, People's Republic of China.
Department of Pharmacy Practice, College of Pharmacy, PAHO/WHO Collaborating Center for Traditional Medicine, University of Illinois at Chicago, Chicago, IL, USA.
Diabetes Metab Syndr Obes. 2020 Apr 1;13:979-989. doi: 10.2147/DMSO.S228674. eCollection 2020.
To evaluate the effect LMHFV on body weight gain, NAFLD and muscle strength and explore effect in mitochondrial biogenesis, AMPKα and p38 pathways.
Vibration platform used in this study provides specific whole-body cyclic mechanical stimulation at low magnitude (0.3 g) and high frequency (50 Hz). Diabetic mice (8-9 mice per group) (C57BL/KsJ-m+/+Lepr) were randomly divided into untreated group (no vibration) and two vibration groups. Lean mice (8 mice) were used as non-diabetic control for both groups. Two diabetic vibration groups received LMHFV every day for 20 min/day and 40 min/day separately.
After 8 weeks of treatment, results showed that body weight, liver weight, fat pad weight, glucose level and insulin level were lower in vibration group when compared with the untreated group. The ratio of fat in liver was significantly decreased after vibration treatment. Muscle strength was significantly increased after vibration. Mitochondrial biogenesis-related gene expression was increased in soleus, gastrocnemius and liver. AMPKα mRNA expression level was increased in soleus and gastrocnemius after vibration treatment. p38 and AMPKα mRNA expression level and protein expression level in liver were enhanced with vibration treatment. Moreover, phosphorylation of p38 and AMPKα was enhanced in liver.
LMHFV applied in our study decreases body weight gain and improves muscle strength and NAFLD in diabetic mice which were partly through improving mitochondrial biogenesis by enhancing p38 and AMPKα pathway.
评估低强度高频振动（LMHFV）对体重增加、非酒精性脂肪性肝病（NAFLD）和肌肉力量的影响，并探讨其在线粒体生物发生、AMPKα和p38信号通路中的作用。
本研究中使用的振动平台可提供低强度（0.3g）和高频（50Hz）的特定全身周期性机械刺激。将糖尿病小鼠（每组8 - 9只小鼠）（C57BL/KsJ-m+/+Lepr）随机分为未处理组（无振动）和两个振动组。瘦小鼠（8只）作为两组的非糖尿病对照。两个糖尿病振动组分别每天接受20分钟/天和40分钟/天的LMHFV。
治疗8周后，结果显示与未处理组相比，振动组的体重、肝脏重量、脂肪垫重量、血糖水平和胰岛素水平较低。振动治疗后肝脏中的脂肪比例显著降低。振动后肌肉力量显著增加。比目鱼肌、腓肠肌和肝脏中线粒体生物发生相关基因表达增加。振动治疗后比目鱼肌和腓肠肌中AMPKα mRNA表达水平增加。振动治疗可增强肝脏中p38和AMPKα mRNA表达水平及蛋白表达水平。此外，肝脏中p38和AMPKα的磷酸化增强。
本研究中应用的LMHFV可降低糖尿病小鼠的体重增加，并改善肌肉力量和NAFLD，这部分是通过增强p38和AMPKα信号通路来改善线粒体生物发生实现的。
