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长链非编码RNA NEAT1/miR-338-3p轴通过调控CREBRF抑制急性髓系白血病的进展。

Long non-coding RNA NEAT1/miR-338-3p axis impedes the progression of acute myeloid leukemia via regulating CREBRF.

作者信息

Feng Song, Liu Na, Chen Xiaoguang, Liu Yufeng, An Jindou

机构信息

Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Erqi District, Zhengzhou, 450052 Henan China.

出版信息

Cancer Cell Int. 2020 Apr 7;20:112. doi: 10.1186/s12935-020-01182-2. eCollection 2020.

DOI:10.1186/s12935-020-01182-2
PMID:32280304
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7137299/
Abstract

BACKGROUND

Acute myeloid leukemia (AML) is a heterogeneous hematological disease. Our purpose of the research was to investigate the regulatory influence of long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1)/microRNA-338-3p (miR-338-3p)/CREB3 regulatory factor (CREBRF) in AML progression.

METHODS

The associated RNA and protein levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot, respectively. Cell growth was assessed through colony formation assay and 3-(4,5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Flow cytometry was exploited to determine the apoptosis rate. Cell migration and invasion were detected by transwell assay. The combination of miR-338-3p and NEAT1 or CREBRF was analyzed via the dual-luciferase reporter assay.

RESULTS

NEAT1 and CREBRF were down-regulated in AML tissues and cells. NEAT1 up-regulation suppressed cell growth, migration and invasion but enhanced apoptosis of AML cells. Inhibition of CREBRF reverted the NEAT1-induced effects on AML cells. Moreover, NEAT1 directly targeted miR-338-3p and miR-338-3p targeted CREBRF. NEAT1/miR-338-3p could affect cellular behaviors of AML cells via the modulation of CREBRF.

CONCLUSION

NEAT1/miR-338-3p axis repressed the AML progression through regulating CREBRF, which might afford a favorable perspective for the AML treatment molecularly.

摘要

背景

急性髓系白血病（AML）是一种异质性血液疾病。我们的研究目的是探究长链非编码RNA（lncRNA）核富集丰富转录本1（NEAT1）/微小RNA-338-3p（miR-338-3p）/CREB3调节因子（CREBRF）在AML进展中的调控作用。

方法

分别通过定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法检测相关RNA和蛋白质水平。通过集落形成试验和3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）试验评估细胞生长情况。利用流式细胞术测定凋亡率。通过Transwell试验检测细胞迁移和侵袭能力。通过双荧光素酶报告基因试验分析miR-338-3p与NEAT1或CREBRF的结合情况。

结果

NEAT1和CREBRF在AML组织和细胞中表达下调。NEAT1上调可抑制AML细胞的生长、迁移和侵袭，但可增强其凋亡。抑制CREBRF可逆转NEAT1对AML细胞的诱导作用。此外，NEAT1直接靶向miR-338-3p，而miR-338-3p靶向CREBRF。NEAT1/miR-338-3p可通过调节CREBRF影响AML细胞的生物学行为。

结论

NEAT1/miR-338-3p轴通过调节CREBRF抑制AML进展，这可能为AML的分子治疗提供良好的前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/fb648686980f/12935_2020_1182_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/1c598d1fed01/12935_2020_1182_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/195d97f71a9d/12935_2020_1182_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/e2e1da823f07/12935_2020_1182_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/cc3e68f8272c/12935_2020_1182_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/5e00305b396f/12935_2020_1182_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/fb648686980f/12935_2020_1182_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/1c598d1fed01/12935_2020_1182_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/195d97f71a9d/12935_2020_1182_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/e2e1da823f07/12935_2020_1182_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/cc3e68f8272c/12935_2020_1182_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/5e00305b396f/12935_2020_1182_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d025/7137299/fb648686980f/12935_2020_1182_Fig6_HTML.jpg

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本文引用的文献

1
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2
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Biomed Pharmacother. 2020 Jan;121:109248. doi: 10.1016/j.biopha.2019.109248. Epub 2019 Nov 14.
3
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Biomark Med. 2024 Aug 17;18(15-16):685-693. doi: 10.1080/17520363.2024.2389036. Epub 2024 Sep 12.
4
UBE2C: A pan-cancer diagnostic and prognostic biomarker revealed through bioinformatics analysis.UBE2C：通过生物信息学分析发现的一种泛癌诊断和预后生物标志物。
Cancer Rep (Hoboken). 2024 Apr;7(4):e2032. doi: 10.1002/cnr2.2032.
5
Puzzling out the role of LncRNA in hepatocellular carcinoma.探究长链非编码RNA在肝细胞癌中的作用。
Noncoding RNA Res. 2024 Jan 11;9(2):547-559. doi: 10.1016/j.ncrna.2024.01.006. eCollection 2024 Jun.
6
Unraveling NEAT1's complex role in lung cancer biology: a comprehensive review.解析NEAT1在肺癌生物学中的复杂作用：一篇综述
EXCLI J. 2024 Jan 3;23:34-52. doi: 10.17179/excli2023-6553. eCollection 2024.
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8
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