Chakravarthi V Praveen, Ghosh Subhra, Dai Eddie, Pathak Devansh, Rumi M A Karim
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, United States.
Data Brief. 2020 Mar 10;30:105405. doi: 10.1016/j.dib.2020.105405. eCollection 2020 Jun.
Disruption of estrogen receptor beta (ESR2) dysregulates granulosa cell genes essential for follicle maturation and ovulation. The datasets presented in this article depict gonadotropin-induced genes, which are differentially expressed in -null rat granulosa cells. Synchronized follicle development was initiated in four-week-old wildtype and -null female rats by administration of PMSG. Forty-eight hours after PMSG injection, further maturation of ovarian follicles was induced by hCG treatment. Granulosa cells were collected from the ovaries before gonadotropin administration, 48 h after PMSG treatment, and 4 h after hCG injection to the PMSG-treated rats. Total RNA was purified from granulosa cells and whole transcriptome was assessed by RNA-sequencing on an Illumina HiSeq X platform. RNA-seq data of wildtype and -null granulosa cells were analyzed and differentially expressed genes were identified by CLC Genomics Workbench. Gonadotropin-induced genes were identified by comparing the transcriptome data of PMSG- or hCG-induced wildtype granulosa cells with those without gonadotropin treatment. Furthermore, differentially expressed genes in -null granulosa cells were determined by comparing the transcriptome data with that of wildtype granulosa cells. These datasets can be used to recognize the gonadotropin-induced genes in granulosa cells that are -regulated and important for ovarian follicle maturation.
雌激素受体β（ESR2）的破坏会使卵泡成熟和排卵所必需的颗粒细胞基因失调。本文呈现的数据集描绘了促性腺激素诱导的基因，这些基因在ESR2基因敲除大鼠的颗粒细胞中差异表达。通过给予孕马血清促性腺激素（PMSG），在四周龄的野生型和ESR2基因敲除雌性大鼠中启动同步卵泡发育。PMSG注射48小时后，通过人绒毛膜促性腺激素（hCG）处理诱导卵巢卵泡进一步成熟。在给予促性腺激素之前、PMSG处理48小时后以及对接受PMSG处理的大鼠注射hCG 4小时后，从卵巢中收集颗粒细胞。从颗粒细胞中纯化总RNA，并在Illumina HiSeq X平台上通过RNA测序评估全转录组。分析野生型和ESR2基因敲除颗粒细胞的RNA-seq数据，并通过CLC基因组工作台鉴定差异表达基因。通过将PMSG或hCG诱导的野生型颗粒细胞的转录组数据与未接受促性腺激素处理的细胞的转录组数据进行比较，鉴定促性腺激素诱导的基因。此外，通过将转录组数据与野生型颗粒细胞的转录组数据进行比较，确定ESR2基因敲除颗粒细胞中的差异表达基因。这些数据集可用于识别颗粒细胞中受ESR2调节且对卵巢卵泡成熟重要的促性腺激素诱导基因。
