Khristi Vincentaben, Chakravarthi V Praveen, Singh Prabhakar, Ghosh Subhra, Pramanik Archit, Ratri Anamika, Borosha Shaon, Roby Katherine F, Wolfe Michael W, Rumi M A Karim
Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS 66160, United States.
Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS 66160, United States.
Data Brief. 2018 May 31;19:1008-1011. doi: 10.1016/j.dib.2018.05.098. eCollection 2018 Aug.
RNA seq analyses were performed in granulosa cells (GCs) collected from gonadotropin treated ESR2 mutant rats. Data obtained from a null mutant with exon 3 deletion (∆3) and another DNA binding domain (DBD) mutant with exon 4 deletion (∆4) were compared to that of wildtype (WT) rats. The raw data were analyzed using CLC genomics workbench. High quality RNA-sequencing reads were aligned to the genome. Differentially expressed genes in ∆3 or ∆4 -mutant GCs were identified based on the following criteria: FDR p-Value ≤0.05 and an absolute fold change of 2. Fewer differentially expressed genes were identified in ∆3 compared to the ∆4 mutant group. As both mutant groups demonstrated a common phenotype of ovulation failure, differentially expressed genes common to both in ∆3 and ∆4 mutant rats were emphasized and further analyzed in the companion article "ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation" [1].
对从经促性腺激素处理的ESR2突变大鼠收集的颗粒细胞（GCs）进行RNA测序分析。将从外显子3缺失的无效突变体（∆3）和另一个外显子4缺失的DNA结合域（DBD）突变体获得的数据与野生型（WT）大鼠的数据进行比较。使用CLC基因组学工作台分析原始数据。高质量的RNA测序读数与基因组进行比对。基于以下标准鉴定∆3或∆4突变体GCs中的差异表达基因：FDR p值≤0.05且绝对倍数变化≥2。与∆4突变体组相比，∆3中鉴定出的差异表达基因较少。由于两个突变体组都表现出排卵失败的共同表型，因此在配套文章《ESR2调节卵泡成熟和排卵所必需的颗粒细胞基因》[1]中强调并进一步分析了∆3和∆4突变大鼠中两者共有的差异表达基因。
