Kumar Amit, Gahlawat S K, Singh Vineeta
ICMR-National Institute of Malaria Research, New Delhi, 10077, India.
Department of Biotechnology, Chaudhary Devi Lal University, Haryana, 125055, India.
Heliyon. 2020 Apr 6;6(4):e03715. doi: 10.1016/j.heliyon.2020.e03715. eCollection 2020 Apr.
Molecular surveillance of the drug resistance genes in parasite can be used for monitoring/surveillance of drug resistance in endemic malaria areas. Here we report the prevalence of single nucleotide polymorphisms (SNPs) in dihydrofolate reductase () gene in nucleotide sequence of Plasmodium falciparum from different regions in India. We found markedly prevalent mutants evident in P. falciparum infections N51I, C59R, 108N and I164L. Our results indicate that P. falciparum populations in the regions show an increase in the prevalence of polymorphisms, most likely reflecting different selective pressures found in humans and mosquitoes. Molecular surveillance can serve as a useful tool to monitor the prevalence/emergence of resistant genotypes within endemic populations and can serve for determining the efficacy of antimalarial drugs.
对寄生虫耐药基因进行分子监测可用于监测/监视疟疾流行地区的耐药情况。在此，我们报告了印度不同地区恶性疟原虫核苷酸序列中二氢叶酸还原酶（）基因的单核苷酸多态性（SNP）的流行情况。我们发现，在恶性疟原虫感染中，N51I、C59R、108N和I164L等突变明显普遍存在。我们的结果表明，这些地区的恶性疟原虫种群中多态性的流行率有所增加，这很可能反映了在人类和蚊子中发现的不同选择压力。分子监测可作为一种有用的工具，用于监测流行人群中耐药基因型的流行情况/出现情况，并可用于确定抗疟药物的疗效。
