de Barros André N C, Santos Emanoela F Q, Rodrigues Dasciana S, Giordano Raquel L C, de Pádua Thiago F
Graduate Program in Chemical Engineering, Federal University of São Carlos, P.O. Box 676, São Carlos, São Paulo 13565-905, Brazil.
Chemical Engineering Department, Federal University of São Carlos, P.O. Box 676, São Carlos, São Paulo 13565-905, Brazil.
ACS Omega. 2020 Mar 23;5(13):7316-7325. doi: 10.1021/acsomega.9b04175. eCollection 2020 Apr 7.
The hydrophobic adsorption is an alternative to traditional organic solvent extraction for the recovery and purification of Penicillin G (PenG). However, there is a lack of information concerning the effect of process variables and technical feasibility while balancing product degradation. After assessing the integrity of PenG under different conditions, Amberlite XAD-4 was selected from among three different adsorbents. During the batch process using only 0.05 g/mL, the adsorption yield increased from 36% at pH 6 to 44% at pH 4. More than 90% of the antibiotic was captured from the fermentation broth using 0.083 g/mL in a 45 min batch performed at pH 4 and 4 °C. Moreover, there was no PenG degradation. The desorption conditions were evaluated, and 95% of the antibiotic could be recovered in only one batch using water-ethanol, which is an unexplored PenG desorption process. The results showed selective adsorption, indicating that the process can also be useful for purification purposes. Hydrophobic adsorption with ethanol desorption is efficient, scalable, and green and could be used in place of traditional methods or in extractive fermentation.
疏水吸附是一种用于青霉素G（PenG）回收和纯化的传统有机溶剂萃取替代方法。然而，在平衡产物降解的同时，缺乏关于工艺变量影响和技术可行性的信息。在评估了不同条件下PenG的完整性后，从三种不同的吸附剂中选择了Amberlite XAD-4。在仅使用0.05 g/mL的间歇过程中，吸附产率从pH 6时的36%增加到pH 4时的44%。在pH 4和4°C下进行的45分钟间歇过程中，使用0.083 g/mL从发酵液中捕获了超过90%的抗生素。此外，没有PenG降解。对解吸条件进行了评估，使用水-乙醇在仅一批次中就可以回收95%的抗生素，这是一种未探索的PenG解吸过程。结果显示出选择性吸附，表明该过程也可用于纯化目的。乙醇解吸的疏水吸附高效、可扩展且环保，可用于替代传统方法或用于萃取发酵。
