He Yongfeng, Wu Xingbing, Zhu Yongjiu, Yang Deguo
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Donghu Hi-Tech Development Zone, Wuhan, Hubei 430223, China.
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 8, Donghu Hi-Tech Development Zone, Wuhan, Hubei 430223, China,
Zoolog Sci. 2020 Apr;37(2):140-147. doi: 10.2108/zs190054.
To elucidate the role of in sex differentiation of a teleost fish , the full-length sequences of its cDNA and promoter were cloned by rapid amplification of cDNA ends (RACE) and genome walking. The relative mRNA expression levels were determined by quantitative real-time PCR (RT-PCR). The 1095-bp cDNA was predicted to encode a protein of 264 amino acids. It was expressed only in the gonads, and the expression was 17-times higher in the testis than in the ovary. The 1215-bp promoter sequence of was cloned and analyzed to detect sex-related differences in its methylation levels. A significant negative relationship between the expression and CpG methylation of its promoter were found in the testes and ovaries of . Significant differences in expression levels were also found between the larval and juvenile stages. No significant differences in expression were found during the entire larval stage, and in the individuals among three different temperature groups (10°C, 14°C, and 18°C). Considering that the sex of sampled larval fish cannot be distinguished, correlations between expression and effects of temperature on sex differentiation in need further study.
为阐明[具体基因名称未给出]在一种硬骨鱼性别分化中的作用，通过cDNA末端快速扩增（RACE）和基因组步移技术克隆了其cDNA和启动子的全长序列。采用实时定量PCR（RT-PCR）测定相对mRNA表达水平。预测1095bp的[具体基因名称未给出]cDNA编码一个264个氨基酸的蛋白质。它仅在性腺中表达，且在睾丸中的表达比在卵巢中高17倍。克隆并分析了1215bp的[具体基因名称未给出]启动子序列，以检测其甲基化水平的性别相关差异。在[具体鱼名称未给出]的睾丸和卵巢中发现[具体基因名称未给出]表达与其启动子的CpG甲基化之间存在显著的负相关关系。在幼体和幼鱼阶段之间也发现了[具体基因名称未给出]表达水平的显著差异。在整个幼体阶段以及三个不同温度组（10°C、14°C和18°C）的个体中未发现表达的显著差异。鉴于无法区分所采样幼体鱼的性别，[具体基因名称未给出]表达与温度对[具体鱼名称未给出]性别分化的影响之间的相关性需要进一步研究。
