Novikov V V, Trofimova M N, Zhil'tsova M A, Biriukova L Z
Antibiot Khimioter. 1988 Nov;33(11):836-8.
For cultivating hybridomas in the ascitic form there are usually used female mice BALB/c and not male ones. Efficiency of production of monoclonal antibodies with cultivation of the hybridomas in male and female mice BALB/c was studied comparatively. The animals were stimulated to form ascite by administration of the incomplete Freund's adjuvant or 3 per cent peptone with petrolatum oil. Some parameters of the ascite formation were studied: viability of the hybridoma cells, ascitic fluid formation period and volume, hybridoma cell concentration and titers of monoclonal antibodies in the ascitic fluid. In regard to all the parameters studied the male animals were not inferior to the female ones and in case of one of the hybridomas even surpassed them twofold by the volume of the ascitic fluid formed. This is evident of possible using male mice for mass cultivation of hybridoma cells with a purpose of obtaining preparative amounts of monoclonal antibodies in production of immunodiagnostic agents on their basis.
为了以腹水形式培养杂交瘤，通常使用雌性BALB/c小鼠，而非雄性。对在雄性和雌性BALB/c小鼠中培养杂交瘤产生单克隆抗体的效率进行了比较研究。通过给予不完全弗氏佐剂或含凡士林油的3%蛋白胨刺激动物形成腹水。研究了腹水形成的一些参数：杂交瘤细胞的活力、腹水形成期和体积、杂交瘤细胞浓度以及腹水中单克隆抗体的效价。就所有研究的参数而言，雄性动物并不逊于雌性动物，在其中一种杂交瘤的情况下，形成的腹水体积甚至比雌性动物高出两倍。这表明在以其为基础生产免疫诊断试剂时，有可能使用雄性小鼠大规模培养杂交瘤细胞以获得制备量的单克隆抗体。
