一种新型缺失突变导致临床来源的碳青霉烯类耐药大肠埃希菌 405 型同时对黏菌素耐药。
A Novel Deletion Mutation in Contributes to Concurrent Colistin Resistance in Carbapenem-Resistant Escherichia coli Sequence Type 405 of Clinical Origin.
机构信息
Division of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.
出版信息
Antimicrob Agents Chemother. 2020 May 21;64(6). doi: 10.1128/AAC.00220-20.
Abstract
We report the first clinical strain EC3000 with concomitant chromosomal colistin and carbapenem resistance. A novel in-frame deletion, Δ6-11 (RPISLR), in that contributes to colistin resistance was verified using recombinant DNA techniques. Although being less fit than the wild-type (WT) strain or EC3000 revertant (chromosomal replacement of WT in EC3000), a portion of serially passaged EC3000 strains preserving colistin resistance without selective pressure raises the concern for further spread.
摘要
我们报告了首例同时具有染色体多粘菌素和碳青霉烯类耐药性的临床分离株 EC3000。通过重组 DNA 技术证实了导致多粘菌素耐药性的新型框内缺失Δ6-11（RPISLR）。尽管与野生型（WT）菌株或 EC3000 回复突变体（WT 在 EC3000 中的染色体替换）相比，适应性更差，但在没有选择压力的情况下连续传代的 EC3000 菌株的一部分保留了多粘菌素耐药性，这引起了对进一步传播的关注。
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