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一种来自植物病原真菌的三分体 ssDNA 真菌病毒可作为克隆 DNA 和纯化病毒粒子进行感染。

A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions.

机构信息

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

出版信息

Sci Adv. 2020 Apr 3;6(14):eaay9634. doi: 10.1126/sciadv.aay9634. eCollection 2020 Apr.

DOI:10.1126/sciadv.aay9634
PMID:32284975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7138691/
Abstract

Here, we describe a tripartite circular single-stranded (ss) DNA mycovirus, named Fusarium graminearum gemytripvirus 1 (FgGMTV1). The genome of FgGMTV1 comprises three circular ssDNA segments (DNA-A, DNA-B, and DNA-C). Sequence alignments and phylogenetic analyses showed that FgGMTV1 is nested within the family Genomoviridae. We also constructed the first infectious DNA clones of a DNA mycovirus. Our results show that DNA-A and DNA-B are mutually interdependent for their replication and are associated with severely reduced colony growth and hypovirulence. DNA-C relies on DNA-A and DNA-B for replication and is necessary for the recovery of abnormal fungal phenotypes. DNA-C also enhances the accumulation of viral DNA in infected fungi and permits stable colonization and easy transmission via conidia. This is the first multipartite DNA virus isolated from a fungus. Our phylogenetic analyses also suggest that the multipartite genome of FgGMTV1 may have evolved from a monopartite genome of an ancient genomovirus.

摘要

在这里，我们描述了一种三方圆形单链 (ss) DNA 真菌病毒，命名为禾谷镰刀菌 gemytripvirus 1（FgGMTV1）。FgGMTV1 的基因组由三个圆形 ssDNA 片段（DNA-A、DNA-B 和 DNA-C）组成。序列比对和系统发育分析表明，FgGMTV1 嵌套在基因组病毒科内。我们还构建了第一个真菌 DNA 病毒的传染性 DNA 克隆。我们的结果表明，DNA-A 和 DNA-B 相互依赖于其复制，并与严重降低的菌落生长和弱毒力有关。DNA-C 依赖于 DNA-A 和 DNA-B 进行复制，对于恢复异常真菌表型是必要的。DNA-C 还增强了感染真菌中病毒 DNA 的积累，并允许通过分生孢子进行稳定的定植和易于传播。这是首次从真菌中分离出的多组分 DNA 病毒。我们的系统发育分析还表明，FgGMTV1 的多组分基因组可能是从古老基因组病毒的单组分基因组进化而来的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/a428a371de70/aay9634-F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/19b5dcce9c26/aay9634-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/4c8579748e5d/aay9634-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/8d1ab03e38a4/aay9634-F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/a428a371de70/aay9634-F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/19b5dcce9c26/aay9634-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/4c8579748e5d/aay9634-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/8d1ab03e38a4/aay9634-F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e669/7138691/a428a371de70/aay9634-F4.jpg

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