State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Center of Growth Metabolism and Aging, Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, China.
National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Sci Adv. 2020 Apr 8;6(15):eaay1514. doi: 10.1126/sciadv.aay1514. eCollection 2020 Apr.
Dental pulp is critical to maintain the vitality of a tooth. Regeneration of pulpo-dentinal complex is of great interest to treat pulpitis and pulp necrosis. In this study, through three-dimensional spheroid culture, a group of unique multipotent stem cells were identified from mouse dental papilla called multipotent dental pulp regenerative stem cells (MDPSCs). MDPSCs exhibited enhanced osteogenic/odontogenic differentiation capabilities and could form regenerative dentin and neurovascular-like structures that mimicked the native teeth in vivo. Further analysis revealed that CD24a was the bona fide marker for MDPSCs, and their expansion was highly dependent on the expression of a key transcriptional factor, . Last, CD24a cells could be detected in primary dental papilla in mice and human, suggesting that MDPSCs resided in their native niches. Together, our study has identified a previously unidentified group of multipotent pulp regenerative stem cells with defined molecular markers for the potential treatment of pulpitis and pulp necrosis.
牙髓对于维持牙齿活力至关重要。牙髓-牙本质复合体的再生对于治疗牙髓炎和牙髓坏死具有重要意义。在这项研究中，通过三维球体培养，从鼠牙乳头中鉴定出一组称为多能牙髓再生干细胞（MDPSCs）的独特多能干细胞。MDPSCs 表现出增强的成骨/成牙分化能力，并能够形成类似于体内天然牙齿的再生牙本质和类血管神经结构。进一步的分析表明，CD24a 是 MDPSCs 的真正标志物，其扩增高度依赖于关键转录因子的表达。最后，在小鼠和人原发性牙髓乳头中可以检测到 CD24a 细胞，表明 MDPSCs 存在于其天然龛位中。总之，我们的研究鉴定了一组以前未被识别的多能牙髓再生干细胞，具有明确的分子标记物，可用于治疗牙髓炎和牙髓坏死。
