Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, College of Animal Science and Technology, Northeast Agricultural University, Harbin, P. R. China.
Anim Sci J. 2020 Jan-Dec;91(1):e13371. doi: 10.1111/asj.13371.
Protein proteolytic enzymes (Proprotein Convertase, PC) is a Ca -dependent serine protease family, whose main function is to cleave precursors of biologically inactive proteins or peptide chains into active functional molecules. Proprotein convertase subtilisin/kexin type 1 (PCSK1) gene is mainly expressed in nerve and endocrine tissues. In this study, PCSK1 was selected as an important candidate gene for abdominal fat content in broilers. We cloned the exon region of chicken PCSK1 gene and found six single-nucleotide polymorphisms (SNPs). Association analysis was carried out and we found that the polymorphisms of these six SNPs were significantly associated with abdominal fat content in G19 and G20 populations. Five of these SNPs were significantly associated with abdominal fat content in G19 and G20 combined population. The polymorphism of these five SNPs was significantly correlated with the abdominal fat content of AA broilers. Together, our study demonstrated that c.927T>C, c.1880C>T, c.*900G>A, and c.*1164C>T were significantly associated with abdominal fat content in populations used in this study, which means that these SNPs in PCSK1 gene could be used as candidate markers to select lean broiler lines.
蛋白水解酶（Proprotein Convertase，PC）是一种依赖 Ca2+的丝氨酸蛋白酶家族，其主要功能是将生物活性较低的蛋白质或肽链前体切割成具有活性的功能分子。脯氨酸羧肽酶/枯草蛋白酶 Kexin 型 1（PCSK1）基因主要在神经和内分泌组织中表达。在本研究中，PCSK1 被选为肉鸡腹脂含量的重要候选基因。我们克隆了鸡 PCSK1 基因的外显子区域，发现了 6 个单核苷酸多态性（SNPs）。关联分析发现，这 6 个 SNP 的多态性与 G19 和 G20 群体的腹脂含量显著相关。其中 5 个 SNP 与 G19 和 G20 组合群体的腹脂含量显著相关。这 5 个 SNP 的多态性与 AA 肉鸡的腹脂含量显著相关。综上所述，本研究表明 c.927T>C、c.1880C>T、c.*900G>A 和 c.*1164C>T 与本研究中使用的群体的腹脂含量显著相关，这意味着 PCSK1 基因中的这些 SNP 可作为选择瘦肉型肉鸡品系的候选标记。
