Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Harbin, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Harbin, China.
Vet Med Sci. 2021 May;7(3):792-799. doi: 10.1002/vms3.422. Epub 2020 Dec 27.
KLF3 is a member of the Kruppel-like factor (KLF) family of transcription factors, and plays an important role in several biological processes, including adipogenesis, erythropoiesis and B-cell development. The purposes of this study are to search for polymorphisms of KLF3 coding region and to provide functional evidence for abdominal fat in chickens. A total of 168 SNPs in KLF3 coding region were detected in a unique chicken population, the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF). Of which three single nucleotide polymorphisms (g.3452T > C, g.8663A > G and g.10751G > A) were significantly correlated with abdominal fat weight (AFW) and abdominal fat percentage (AFP) of 329 birds from the 19th generation of NEAUHLF (FDR < 0.05). The reporter gene assay was performed to verify functionality of these three SNPs in both ICP-1 and DF1 cells. Results showed that the luciferase activity of G allele was significantly higher than that of A allele in g.10751G > A (p < 0.05). However, there were no significant differences between different alleles of others two SNPs in luciferase activity. Overall, KLF3 is an important candidate gene that affects chicken abdominal fat content, and the g.10751G > A is a functional variant that potential would be applied to marker-assisted selection (MAS) for selective breeding programme.
KLF3 是 Kruppel 样因子（KLF）家族的转录因子成员，在几个生物学过程中发挥重要作用，包括脂肪生成、红细胞生成和 B 细胞发育。本研究的目的是寻找 KLF3 编码区的多态性，并为鸡的腹部脂肪提供功能证据。在一个独特的鸡群体——东北农业大学肉鸡系（NEAUHLF）中，对 KLF3 编码区进行了 168 个 SNP 的检测，该群体对腹部脂肪含量进行了不同的选择。其中三个单核苷酸多态性（g.3452T>C、g.8663A>G 和 g.10751G>A）与 19 代 NEAUHLF 中的 329 只鸡的腹部脂肪重量（AFW）和腹部脂肪百分比（AFP）显著相关（FDR<0.05）。进行了报告基因检测，以验证 ICP-1 和 DF1 细胞中这三个 SNP 的功能。结果表明，g.10751G>A 中 G 等位基因的荧光素酶活性显著高于 A 等位基因（p<0.05）。然而，在其他两个 SNP 的不同等位基因之间，荧光素酶活性没有显著差异。总体而言，KLF3 是一个重要的候选基因，影响鸡的腹部脂肪含量，g.10751G>A 是一个潜在的功能变体，可能会应用于选择育种计划的标记辅助选择（MAS）。
