Tice R R
Medical Department, Brookhaven National Laboratory, Upton, New York 11973.
Cell Biol Toxicol. 1988 Dec;4(4):475-86. doi: 10.1007/BF00117775.
With the growing realization that in vitro short-term tests for genotoxicity can never fully mimic in vivo conditions, the evaluation of genotoxic damage in somatic cells of rodents has played an increasingly important role in assessing the carcinogenic potential of suspect compounds. Among the various genotoxic endpoints assessed in in vivo somatic cell assays, cytogenetic endpoints (e.g., chromosomal aberrations, micronuclei, sister chromatid exchanges) continue to be used most frequently. The purpose of this paper is to demonstrate the utility of evaluating different cytogenetic endpoints in the same animal, using as examples studies to evaluate the in vivo genotoxic potential of benzene, of methylisocyanate, and of butadiene, chloroprene and isoprene.
随着人们越来越意识到遗传毒性的体外短期试验永远无法完全模拟体内情况，对啮齿动物体细胞中遗传毒性损伤的评估在评估可疑化合物的致癌潜力方面发挥着越来越重要的作用。在体内体细胞试验评估的各种遗传毒性终点中，细胞遗传学终点（如染色体畸变、微核、姐妹染色单体交换）仍然是最常用的。本文的目的是通过以评估苯、甲基异氰酸酯、丁二烯、氯丁二烯和异戊二烯的体内遗传毒性潜力的研究为例，证明在同一动物中评估不同细胞遗传学终点的实用性。
