高内涵高通量显微镜技术在药物研发中的潜力。
The potential of high-content high-throughput microscopy in drug discovery.
作者信息
Starkuviene V, Pepperkok R
机构信息
Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, 69117 Heidelberg, Germany.
出版信息
Br J Pharmacol. 2007 Sep;152(1):62-71. doi: 10.1038/sj.bjp.0707346. Epub 2007 Jul 2.
Abstract
Fluorescence microscopy is a powerful method to study protein function in its natural habitat, the living cell. With the availability of the green fluorescent protein and its spectral variants, almost any gene of interest can be fluorescently labelled in living cells opening the possibility to study protein localization, dynamics and interactions. The emergence of automated cellular systems allows rapid visualization of large groups of cells and phenotypic analysis in a quantitative manner. Here, we discuss recent advances in high-content high-throughput microscopy and its potential application to several steps of the drug discovery process.
摘要
荧光显微镜术是一种在活细胞这个天然环境中研究蛋白质功能的强大方法。随着绿色荧光蛋白及其光谱变体的问世，几乎任何感兴趣的基因都能在活细胞中进行荧光标记，从而开启了研究蛋白质定位、动力学及相互作用的可能性。自动化细胞系统的出现使得对大量细胞进行快速可视化观察以及进行定量表型分析成为可能。在此，我们将讨论高内涵高通量显微镜术的最新进展及其在药物发现过程多个步骤中的潜在应用。
相似文献
1
The potential of high-content high-throughput microscopy in drug discovery.高内涵高通量显微镜技术在药物研发中的潜力。
Br J Pharmacol. 2007 Sep;152(1):62-71. doi: 10.1038/sj.bjp.0707346. Epub 2007 Jul 2.
2
High-throughput microscopy must re-invent the microscope rather than speed up its functions.高通量显微镜必须对显微镜进行重新发明，而不是加快其功能。
Br J Pharmacol. 2007 Sep;152(1):1-4. doi: 10.1038/sj.bjp.0707348. Epub 2007 Jul 2.
3
Multidimensional drug profiling by automated microscopy.通过自动显微镜进行多维药物分析
Science. 2004 Nov 12;306(5699):1194-8. doi: 10.1126/science.1100709.
4
Integrated expressional analysis: application to the drug discovery process.综合表达分析：在药物发现过程中的应用。
Methods. 2005 Nov;37(3):280-8. doi: 10.1016/j.ymeth.2005.03.013.
5
Image-based screening: a technology in transition.基于图像的筛查：一项正在转型的技术。
Curr Opin Biotechnol. 2005 Feb;16(1):41-8. doi: 10.1016/j.copbio.2004.12.005.
6
Image-based chemical screening.基于图像的化学筛选
Nat Chem Biol. 2007 Aug;3(8):461-5. doi: 10.1038/nchembio.2007.15.
7
Live cell imaging: an industrial perspective.活细胞成像：行业视角
Methods Mol Biol. 2010;591:47-66. doi: 10.1007/978-1-60761-404-3_3.
8
High-throughput RNAi screening by time-lapse imaging of live human cells.通过对活的人类细胞进行延时成像进行高通量RNA干扰筛选。
Nat Methods. 2006 May;3(5):385-90. doi: 10.1038/nmeth876.
9
Automated image analysis for high-content screening and analysis.用于高内涵筛选和分析的自动化图像分析
J Biomol Screen. 2010 Aug;15(7):726-34. doi: 10.1177/1087057110370894. Epub 2010 May 20.
10
Cellular imaging in drug discovery.药物研发中的细胞成像
Nat Rev Drug Discov. 2006 Apr;5(4):343-56. doi: 10.1038/nrd2008.
引用本文的文献
1
Fourier Ptychographic Microscopy 10 Years on: A Review.傅里叶叠层显微术 10 年进展综述
Cells. 2024 Feb 10;13(4):324. doi: 10.3390/cells13040324.
2
Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth.用于无损定量 3D 细胞培养生长的人骨髓间充质干细胞（hMSCs）容积成像。
PLoS One. 2023 Mar 28;18(3):e0282298. doi: 10.1371/journal.pone.0282298. eCollection 2023.
3
Correlative Multi-Modal Microscopy: A Novel Pipeline for Optimizing Fluorescence Microscopy Resolutions in Biological Applications.相关多模态显微镜：一种优化生物应用中荧光显微镜分辨率的新方案。
Cells. 2023 Jan 17;12(3):354. doi: 10.3390/cells12030354.
4
Generalising from conventional pipelines using deep learning in high-throughput screening workflows.利用深度学习在高通量筛选工作流程中对传统管道进行泛化。
Sci Rep. 2022 Jul 6;12(1):11465. doi: 10.1038/s41598-022-15623-7.
5
Learning the rules of collective cell migration using deep attention networks.使用深度注意网络学习细胞集体迁移的规则。
PLoS Comput Biol. 2022 Apr 27;18(4):e1009293. doi: 10.1371/journal.pcbi.1009293. eCollection 2022 Apr.
6
Monitoring cell endocytosis of liposomes by real-time electrical impedance spectroscopy.实时阻抗谱监测脂质体的细胞内吞作用。
Anal Bioanal Chem. 2020 Sep;412(24):6371-6380. doi: 10.1007/s00216-020-02592-x. Epub 2020 May 25.
7
Quantitative analysis of interactive behavior of mitochondria and lysosomes using structured illumination microscopy.使用结构光照明显微镜对线粒体与溶酶体的相互作用行为进行定量分析。
Biomaterials. 2020 Aug;250:120059. doi: 10.1016/j.biomaterials.2020.120059. Epub 2020 Apr 19.
8
Speckle-structured illumination for 3D phase and fluorescence computational microscopy.用于三维相位和荧光计算显微镜的散斑结构照明
Biomed Opt Express. 2019 Jun 26;10(7):3635-3653. doi: 10.1364/BOE.10.003635. eCollection 2019 Jul 1.
9
Computational structured illumination for high-content fluorescence and phase microscopy.用于高内涵荧光和相显微镜的计算结构照明显微技术。
Biomed Opt Express. 2019 Mar 22;10(4):1978-1998. doi: 10.1364/BOE.10.001978. eCollection 2019 Apr 1.
10
Synthetic 10FN3-based mono- and bivalent inhibitors of MDM2/X function.基于合成的10FN3的MDM2/X功能单价和二价抑制剂。
Protein Eng Des Sel. 2018 Jul 1;31(7-8):301-312. doi: 10.1093/protein/gzy018.
本文引用的文献
1
Reverse transfection on cell arrays for high content screening microscopy.用于高内涵筛选显微镜检查的细胞阵列反向转染。
Nat Protoc. 2007;2(2):392-9. doi: 10.1038/nprot.2006.483.
2
High-content screening of functional genomic libraries.功能基因组文库的高内涵筛选
Methods Enzymol. 2006;414:530-65. doi: 10.1016/S0076-6879(06)14028-8.
3
An infrastructure for high-throughput microscopy: instrumentation, informatics, and integration.高通量显微镜技术的基础设施：仪器设备、信息学与整合
Methods Enzymol. 2006;414:484-512. doi: 10.1016/S0076-6879(06)14026-4.
4
High-content screening: emerging hardware and software technologies.高内涵筛选：新兴的硬件与软件技术
Methods Enzymol. 2006;414:468-83. doi: 10.1016/S0076-6879(06)14025-2.
5
Development and application of automatic high-resolution light microscopy for cell-based screens.用于基于细胞的筛选的自动高分辨率光学显微镜的开发与应用。
Methods Enzymol. 2006;414:228-47. doi: 10.1016/S0076-6879(06)14013-6.
6
High-content microscopy identifies new neurite outgrowth regulators.高内涵显微镜技术鉴定出新的神经突生长调节因子。
Mol Biol Cell. 2007 Jan;18(1):242-52. doi: 10.1091/mbc.e06-08-0666. Epub 2006 Nov 8.
7
Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking.颅-晶状体-缝发育异常由SEC23A突变引起，导致内质网到高尔基体的运输异常。
Nat Genet. 2006 Oct;38(10):1192-7. doi: 10.1038/ng1876. Epub 2006 Sep 17.
8
Secretory COPII coat component Sec23a is essential for craniofacial chondrocyte maturation.分泌型COPII包被组分Sec23a对颅面部软骨细胞成熟至关重要。
Nat Genet. 2006 Oct;38(10):1198-203. doi: 10.1038/ng1880. Epub 2006 Sep 17.
9
shRNA libraries and their use in cancer genetics.短发夹RNA文库及其在癌症遗传学中的应用。
Nat Methods. 2006 Sep;3(9):701-6. doi: 10.1038/nmeth921.
10
High-throughput fluorescence microscopy for systems biology.用于系统生物学的高通量荧光显微镜技术
Nat Rev Mol Cell Biol. 2006 Sep;7(9):690-6. doi: 10.1038/nrm1979. Epub 2006 Jul 19.
Zotero 插件