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严重急性呼吸综合征冠状病毒中开放阅读框3的假定结构与功能

Putative structure and function of ORF3 in SARS coronavirus.

作者信息

Zhang Xue Wu, Yap Yee Leng

机构信息

HKU-Pasteur Research Center, Department of Bioinformatics, 8 Sassoon Road, Pokfulam, Hong Kong, China.

出版信息

Theochem. 2005 Feb 28;715(1):55-58. doi: 10.1016/j.theochem.2004.10.073. Epub 2004 Dec 18.

DOI:10.1016/j.theochem.2004.10.073
PMID:32287548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7113666/
Abstract

Based on molecular modeling techniques we constructed a rational 3D model of ORF3 in SARS coronavirus (SARS-CoV). Our studies suggest that the function of ORF3 could be involved in FAD/NAD binding according to its predicted structure and comparison with other structure neighbors. Furthermore, we identified three pairs of non-canonical N-H⋯π interactions in the structure of ORF3, which can make contributions to the stability of protein structure. These results provide important clues for better understanding of SARS-CoV ORF3 and trying new therapeutic strategies.

摘要

基于分子建模技术，我们构建了严重急性呼吸综合征冠状病毒（SARS-CoV）中开放阅读框3（ORF3）的合理三维模型。我们的研究表明，根据其预测结构以及与其他结构邻域的比较，ORF3的功能可能与黄素腺嘌呤二核苷酸（FAD）/烟酰胺腺嘌呤二核苷酸（NAD）结合有关。此外，我们在ORF3结构中鉴定出三对非经典的N-H⋯π相互作用，它们有助于蛋白质结构的稳定性。这些结果为更好地理解SARS-CoV ORF3及尝试新的治疗策略提供了重要线索。

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