Ding Jun, Liu Qiao-Rong, Han Jin-Peng, Qian Wei-Feng, Liu Qun
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture/College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R. China.
J Integr Agric. 2012 Oct;11(10):1721-1728. doi: 10.1016/S2095-3119(12)60176-2. Epub 2012 Oct 22.
Coccidiosis is caused by intra-cellular infection of spp., which goes through a complex life cycle in the intestinal mucosa of infected hosts. Specific immunoglobulins (IgY) could be produced in egg yolk by immunizing hens with specific antigens. In the present study, we cloned the gene, expressed the GST-GAM56 fusion protein and raised IgY to GST-GAM56 in hens. The anti-GST-GAM56 IgY antibody was isolated and used to treat chickens infected with oocysts. Intramuscular injection of the antibodies provided minimal protection against parasite infection. However, oral dosing of the IgY 3 or 5 d after oocyst inoculation significantly improved body weight gain, reduced oocyst output and intestinal lesion score were reduced at 3 or 5 d after oocyst challenging, compared to the untreated control group. Our findings suggest that the IgY to gam56 could be an effective prophylactic or therapeutic agent against infection in chickens and should have a practical application value.
球虫病由 属的细胞内感染引起，其在受感染宿主的肠黏膜中经历复杂的生命周期。通过用特定抗原免疫母鸡，可在蛋黄中产生特异性免疫球蛋白（IgY）。在本研究中，我们克隆了 基因，表达了GST - GAM56融合蛋白，并在母鸡中制备了针对GST - GAM56的IgY。分离出抗GST - GAM56 IgY抗体并用于治疗感染了 卵囊的鸡。肌肉注射该抗体对寄生虫感染提供的保护作用极小。然而，与未处理的对照组相比，在卵囊接种后3或5天口服IgY可显著改善体重增加，减少卵囊排出量，并且在卵囊攻击后3或5天肠道病变评分降低。我们的研究结果表明，针对gam56的IgY可能是预防或治疗鸡感染 的有效制剂，具有实际应用价值。
