Lee S H, Lillehoj H S, Park D W, Jang S I, Morales A, García D, Lucio E, Larios R, Victoria G, Marrufo D, Lillehoj E P
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, USDA, Beltsville, MD 20705, USA.
Poult Sci. 2009 Mar;88(3):562-6. doi: 10.3382/ps.2008-00340.
The protective effect of hyperimmune IgY fraction of egg yolk prepared from hens hyperimmunized with multiple species of Eimeria oocysts on experimental coccidiosis was evaluated in young broilers. Chickens were continuously fed from hatch with a standard diet containing hyperimmune IgY egg yolk powder or a nonsupplemented control diet and orally challenged at d 7 posthatch with 5.0 x 10(3) sporulated Eimeria acervulina oocysts. Body weight gain between d 0 and 10 and fecal oocyst shedding between d 5 and 10 postinfection were determined as parameters of protective immunity. Chickens given 10 or 20% hyperimmune IgY egg yolk powder showed significantly increased BW gain and reduced fecal oocyst shedding compared with control birds fed the nonsupplemented diet. In another trial, lower IgY concentrations (0.01, 0.02, and 0.05%) were used to treat birds with 1.0 x 10(4) oocysts of E. acervulina. Total oocyst shedding was significantly (P < 0.05) reduced in chickens fed the 0.02 and 0.05% hyperimmune IgY supplemented-diets compared with animals fed the nonsupplemented diet. Similarly, chickens fed 0.5% of hyperimmune IgY egg yolk powder diet and challenged with 1.0 x 10(4) oocysts exhibited reduced oocyst shedding compared with the control birds given 0.5% of IgY from nonimmunized hen eggs, although BW gain was not affected. We conclude that passive immunization of chickens with anti-coccidia IgY antibodies provide protective immunity against coccidiosis challenge infection.
在幼龄肉鸡中评估了用多种艾美耳球虫卵囊对母鸡进行超免疫制备的蛋黄超免疫IgY组分对实验性球虫病的保护作用。从孵化开始，给鸡持续饲喂含有超免疫IgY蛋黄粉的标准日粮或未添加的对照日粮，并在孵化后第7天口服接种5.0×10³个孢子化的堆型艾美耳球虫卵囊。将0至10日龄期间的体重增加以及感染后第5至10天的粪便卵囊排出量作为保护性免疫的参数。与饲喂未添加日粮的对照鸡相比，给予10%或20%超免疫IgY蛋黄粉的鸡体重增加显著增加，粪便卵囊排出量减少。在另一项试验中，使用较低浓度的IgY（0.01%、0.02%和0.05%）来处理接种1.0×10⁴个堆型艾美耳球虫卵囊的鸡。与饲喂未添加日粮的动物相比，饲喂添加0.02%和0.05%超免疫IgY日粮的鸡的总卵囊排出量显著（P<0.05）减少。同样，饲喂0.5%超免疫IgY蛋黄粉日粮并接种1.0×10⁴个卵囊的鸡与给予0.5%未免疫母鸡鸡蛋IgY的对照鸡相比，卵囊排出量减少，尽管体重增加未受影响。我们得出结论，用抗球虫IgY抗体对鸡进行被动免疫可提供针对球虫病攻击感染的保护性免疫。
