利用热水使猪流行性腹泻病毒失活。
Inactivation of porcine epidemic diarrhea virus using heated water.
作者信息
Zentkovich Michele M, Nelson Sarah W, Stull Jason W, Nolting Jacqueline M, Bowman Andrew S
机构信息
The Ohio State University, 1920 Coffey Road, Columbus, OH 43210, USA.
出版信息
Vet Anim Sci. 2016 Dec;1:1-3. doi: 10.1016/j.vas.2016.09.001. Epub 2016 Sep 13.
Abstract
Porcine epidemic diarrhea virus (PEDV) is a very contagious swine pathogen that spreads easily via the fecal-oral route, notably from contaminated fomites. The present study investigated heated water as a method for rapid thermal inactivation of PEDV. Cell-culture adapted PEDV was treated with water at varying temperatures and viral titers were measured at multiple time points post-treatment. Viable PEDV was not recovered after a ten second or longer treatment with water heated to ≥76 °C; however, PEDV nucleic acid was detected in all samples regardless of treatment. Hot water decontamination could be considered in settings where chemical disinfection is impractical.
摘要
猪流行性腹泻病毒（PEDV）是一种极具传染性的猪病原体，很容易通过粪口途径传播，尤其是通过受污染的污染物传播。本研究调查了热水作为一种快速热灭活PEDV的方法。将适应细胞培养的PEDV用不同温度的水进行处理，并在处理后的多个时间点测量病毒滴度。用加热至≥76°C的水进行10秒或更长时间的处理后，未检测到活的PEDV；然而，无论处理如何，在所有样品中均检测到PEDV核酸。在化学消毒不切实际的情况下，可以考虑采用热水去污。
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