State Key Laboratory of Crop Stress Adaptation and Improvement, State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng 475004, Henan, China.
Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
Plant Dis. 2020 Jun;104(6):1709-1714. doi: 10.1094/PDIS-11-19-2431-RE. Epub 2020 Apr 14.
Powdery mildew, caused by the biotrophic fungal pathogen f. sp. (), is a globally important wheat disease causing severe yield losses, and deployment of resistant varieties is the preferred choice for managing this disease. Chinese wheat landrace Datoumai was resistant to 22 of 23 isolates at the seedling stage. Genetic analysis based on the inoculation of isolate E09 on the F, F, and F populations derived from the cross Datoumai × Huixianhong revealed that the powdery mildew resistance of Datoumai is controlled by a single dominant gene, temporarily designated as . Bulked segregant analysis and simple sequence repeat mapping with 200 F plants showed that was located in the same genetic region as on chromosome 1DS. To fine map , 12 critical recombinants were identified from 1,192 F plants and delimited to a 0.5-cM to interval covering 180.5 Kb (38,728,125 to 38,908,656 bp) on chromosome 1DS, and only one highly confident gene, , was annotated within this region. encodes a receptor-like serine/threonine-protein kinase (STK), and a 6-bp deletion in exon 5 may confer the resistance to powdery mildew. Allele frequency analysis indicated that the allele with 6-bp deletion was only present in three landraces (Datoumai, Chiyacao [], and Hulutou) and was absent in all of the 353 Chinese modern cultivars and 147 foreign cultivars. These results demonstrate that is mapped to the same locus as and can be widely used to enhance powdery mildew resistance in wheat growing regions worldwide.
小麦白粉病由专性寄生真菌 f. sp. 引起，是一种全球性的重要小麦病害，可导致严重的产量损失，而利用抗性品种是防治该病害的首选。中国小麦地方品种大头麦在幼苗期对 23 个分离物中的 22 个具有抗性。基于对分离物 E09 在大头麦×辉县红杂交后代的 F 、 F 和 F 群体接种的遗传分析表明，大头麦的白粉病抗性由单个显性基因控制，暂命名为 。利用 200 个 F 植株进行的混池分离分析和简单序列重复图谱构建表明， 与染色体 1DS 上的 位于相同的遗传区域。为了精细定位 ，从 1192 个 F 植株中鉴定出 12 个关键重组体，并将其限定在一个 0.5-cM 至 区间内，包含染色体 1DS 上的 180.5 Kb（38728125bp 至 38908656bp），该区域内仅注释了一个高度置信的基因 。 编码一个受体样丝氨酸/苏氨酸蛋白激酶（STK），外显子 5 中的 6-bp 缺失可能赋予其对白粉病的抗性。等位基因频率分析表明，缺失 6-bp 的 等位基因仅存在于三个地方品种（大头麦、赤崖藻和葫芦头）中，而在所有 353 个中国现代品种和 147 个外国品种中均不存在。这些结果表明， 被定位到与 相同的位点上，可广泛用于提高世界各地小麦种植区对白粉病的抗性。
