Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Banatska 31b, 11080, Belgrade, Serbia.
Agency for Medicines and Medical Devices of Montenegro, Podgorica, Montenegro.
Chem Biol Interact. 2020 Jun 1;324:109084. doi: 10.1016/j.cbi.2020.109084. Epub 2020 Apr 11.
An imbalance between oxidants and antioxidants in favour of oxidants, potentially leading to damage, is termed oxidative stress. Antioxidants (AO), either enzymatic or non-enzymatic, are the ones that can reduce diverse effects of pro-oxidants such as DNA, proteins and lipids damage. Chalcones (1,3-diaryl-2-propen-1-ones) are open chain flavonoids that are widely biosynthesized in plants. Aim of this study was to test antioxidative potency of 15 chalcones (Chs) in in vitro model in serum (native conditions), so as with exogenously induced oxidative stress.
Oxidative stress was induced in serum samples of healthy individuals with 0.25 mmol/L terc-buthyl-hydroperoxide (TBH), and then we monitored the effects of various concentrations of chalcones on oxidative stress parameters: total antioxidative status (TAS), total oxidative status (TOS), total concentration of sulfhydryl group (SHG) and prooxidative-antioxidative balance (PAB), and calculated prooxidative score, antioxidative score, and oxy score (OS). Nonparametric repeated measures ANOVA (Friedman's test) was used for comparison of antioxidative potency of samples with 15 different chalcones, in a native state and upon TBH influence. Spearman's nonparametric correlation analysis was used for estimation of relation between different parameters.
Negative Oxy Score (OS) values for Chs11-15 showed significantly stronger antioxidative potency compared to other investigated chalcones (p < 0.05). Ch11, Ch13 and Ch14 remained with negative OS even after TBH addition, whereas OS of Ch12 and Ch15 became positive, with small nominal values. Samples with Ch11 and Ch13 showed significant negative correlation between TAS and TOS (p < 0.05 for both), but in Ch14 sample the negative correlation existed between TAS and PAB (p < 0.05).
Lower value of OS (i.e. better antioxidative potency) was noticed in samples with Ch11-Ch15. Electron-donor effects of substituent groups as a structural part of these chalcones could explain its antioxidative capability. Phenolic and methyl groups are responsible for antioxidative ability enhancement of five chalcones with the best activity.
氧化剂与抗氧化剂之间的平衡倾向于氧化剂，可能导致损伤，这种情况被称为氧化应激。抗氧化剂（AO），无论是酶促的还是非酶促的，都可以减少自由基如 DNA、蛋白质和脂质损伤等多种作用。查耳酮（1,3-二芳基-2-丙烯-1-酮）是广泛存在于植物中的开链类黄酮。本研究旨在测试 15 种查耳酮（Chs）在血清中的体外模型中的抗氧化能力（在天然条件下），以及在体外诱导氧化应激时的抗氧化能力。
用 0.25mmol/L 叔丁基过氧化氢（TBH）诱导健康个体血清中的氧化应激，然后监测各种浓度查耳酮对氧化应激参数的影响：总抗氧化状态（TAS）、总氧化状态（TOS）、总巯基浓度（SHG）和促氧化剂-抗氧化剂平衡（PAB），并计算促氧化剂评分、抗氧化剂评分和氧评分（OS）。采用非参数重复测量方差分析（Friedman 检验）比较 15 种不同查耳酮在天然状态和 TBH 影响下的抗氧化能力。采用 Spearman 非参数相关分析估计不同参数之间的关系。
Chs11-15 的负氧评分（OS）值表明其抗氧化能力明显强于其他研究的查耳酮（p<0.05）。Ch11、Ch13 和 Ch14 在加入 TBH 后仍保持负 OS，而 Ch12 和 Ch15 的 OS 变为正，数值较小。Ch11 和 Ch13 样品的 TAS 和 TOS 之间呈显著负相关（两者均 p<0.05），但 Ch14 样品中 TAS 和 PAB 之间呈负相关（p<0.05）。
Ch11-Ch15 样品的 OS 值较低（即抗氧化能力较强）。作为这些查耳酮结构部分的取代基的供电子效应可以解释其抗氧化能力。酚基和甲基基团增强了 5 种具有最佳活性的查耳酮的抗氧化能力。
