Pharmaceutical Technology Department, National Research Centre, Cairo, Egypt.
Cellulose and Paper Department, National Research Centre, Cairo, Egypt.
Int J Biol Macromol. 2020 Aug 1;156:704-716. doi: 10.1016/j.ijbiomac.2020.04.019. Epub 2020 Apr 11.
This study intended to design novel nanofibrillated cellulose/cyclodextrin-based 3D scaffolds loaded with raloxifene hydrochloride for bone regeneration. The scaffolds were prepared using two different types of cyclodextrins namely; beta-cyclodextrin and methyl-beta-cyclodextrin. The prepared scaffolds were evaluated by characterizing their porosity, compressive strength, in-vitro drug release, FT-IR and XRD as well as their morphological properties using SEM. Results presented that the prepared scaffolds were highly porous, additionally, the scaffold containing drug/beta-cyclodextrin kneaded complex (SC5) showed the most controlled drug release pattern with the least burst effect and reached almost complete release at 480 h. The in-vitro cytocompatibility and regenerative effect of the chosen scaffold (SC5) was assessed using Saos-2 cell line. Results proved that SC5 was biocompatible. Moreover, it enhanced the cell adhesion, alkaline phosphatase enzyme expression and calcium ion deposition which are essential factors for bone mineralization. The obtained observations presented a novel, safe and propitious approach for bone engineering.
本研究旨在设计新型纳米原纤化纤维素/环糊精基 3D 支架，负载盐酸雷洛昔芬用于骨再生。支架采用两种不同类型的环糊精，即β-环糊精和甲基-β-环糊精制备。通过对孔隙率、压缩强度、体外药物释放、FT-IR 和 XRD 进行评价，并使用 SEM 观察其形态特性，对制备的支架进行了评价。结果表明，所制备的支架具有很高的孔隙率，此外，含有药物/β-环糊精捏合复合物（SC5）的支架显示出最可控的药物释放模式，具有最小的突释效应，在 480 小时内几乎完全释放。选择的支架（SC5）的体外细胞相容性和再生效果使用 Saos-2 细胞系进行评估。结果证明 SC5 具有生物相容性。此外，它增强了细胞黏附、碱性磷酸酶表达和钙离子沉积，这些都是骨矿化的必要因素。获得的观察结果为骨工程提供了一种新颖、安全和有利的方法。
