College of Food Science & Engineering, Ocean University of China, 5th Yushan Road, Qingdao, Shandong 266003, China.
Department of Marine Life Sciences, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province 63333, Republic of Korea.
Int J Biol Macromol. 2020 Aug 1;156:717-729. doi: 10.1016/j.ijbiomac.2020.04.012. Epub 2020 Apr 11.
A fucoidan (LJSF4) purified from Saccharina japonica was found to show a strong anti-inflammatory effect via activity assay in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells and zebrafish. Chemical and structural analysis indicated that LJSF4 with a sulfate content of 30.72% was composed of fucose, galactose, rhamnose, xylose and mannose with molar ratio percentages of 79.49%, 16.76%, 0.82%, 1.08% and 1.84%. NMR spectroscopy showed that LJSF4 is a polysaccharide with a backbone of alternating 1 → 3 linked α-l-fucopyranosyl and →4-α-l-fucopyranosyl with sulfate groups mainly at C-4 and partially at C-2 positions. Moreover, it also contained branches in the form of β-d-Galp-(1 → 4) units. The results of anti-inflammatory effect in vitro demonstrated that LJSF4 decreased the production of nitric oxide (NO) and cytokines, including TNF-α, IL-1β and IL-6. The mechanism revealed to be associated with the down-regulated expression of signal pathways including MAPK and NF-κB. By in vivo assay, LJSF4 showed a significantly protective effect by reducing the cell death rate, and the production of NO and ROS on LPS exposed zebrafish. Our results indicated that LJSF4 has the potential to be developed as an anti-inflammatory agent applied in functional food and cosmetic industries.
从褐藻中提取的岩藻聚糖（LJSF4）通过脂多糖（LPS）诱导的 RAW 264.7 巨噬细胞和斑马鱼的活性测定显示出很强的抗炎作用。化学和结构分析表明，LJSF4 硫酸基含量为 30.72%，由岩藻糖、半乳糖、鼠李糖、木糖和甘露糖组成，摩尔比分别为 79.49%、16.76%、0.82%、1.08%和 1.84%。NMR 光谱表明，LJSF4 是一种具有交替 1→3 连接的α-L-岩藻糖和→4-α-L-岩藻糖主链的多糖，硫酸基主要位于 C-4 位，部分位于 C-2 位。此外，它还含有β-d-Galp-（1→4）单元形式的支链。体外抗炎作用结果表明，LJSF4 降低了一氧化氮（NO）和细胞因子（TNF-α、IL-1β 和 IL-6）的产生。其机制与 MAPK 和 NF-κB 信号通路的下调表达有关。通过体内试验，LJSF4 对 LPS 暴露的斑马鱼具有显著的保护作用，降低了细胞死亡率以及 NO 和 ROS 的产生。我们的研究结果表明，LJSF4 具有作为抗炎剂应用于功能性食品和化妆品行业的潜力。
