Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania.
Department of Physics, Chemistry and Biology, Linköping University, 58183 Linköping, Sweden.
Colloids Surf B Biointerfaces. 2020 Jul;191:110999. doi: 10.1016/j.colsurfb.2020.110999. Epub 2020 Mar 27.
In this research a whispering gallery mode (WGM) resonator based on vertically oriented ZnO nanorods, which were formed on silicon surface (silicon/ZnO-NRs), has been applied in the design of optical immunosensor that was dedicated for the determination of grapevine virus A-type (GVA) proteins. Vertically oriented ZnO-NRs were grown on silicon substrates by atmospheric pressure metal organic chemical vapor deposition (APMOCVD) and the silicon/ZnO-NRs structures formed were characterized by structural and optical methods. Optical characterization demonstrates that silicon/ZnO-NRs-based structures can act as 'whispering gallery mode' (WGM) resonator where quasi-whispering gallery modes (quasi-WGMs) are generated. These quasi-WGMs were experimentally observed in the visible and infrared ranges of the photoluminescence spectra. In order to design an immuno-sensing system the anti-GVA antibodies were immobilized on the surface of silicon/ZnO-NRs and in this way silicon/ZnO-NRs/anti-GVA structure was formed. The immobilization of anti-GVA antibodies and then the interaction of silicon/ZnO-NRs/anti-GVA structure with GVA proteins (GVA-antigens) resulted in an opposite shifts of the WGMs peaks in the visible range of the photoluminescence spectra observed as a defect-related photoluminescence emission of ZnO-NRs. Here designed silicon/ZnO-NRs/anti-GVA immuno-sensing structure demonstrates the sensitivity towards GVA-antigens in the concentration range of 1-200 ng/ml. Bioanalytical applicability of the silicon/ZnO-NRs-based structures in the WGMs registration mode is discussed.
在这项研究中，基于垂直取向的 ZnO 纳米棒的 whispering gallery 模式（WGM）谐振器被应用于设计用于测定葡萄病毒 A 型（GVA）蛋白的光学免疫传感器。垂直取向的 ZnO 纳米棒通过常压金属有机化学气相沉积（APMOCVD）生长在硅衬底上，并用结构和光学方法对形成的硅/ZnO-NRs 结构进行了表征。光学特性表明，硅/ZnO-NRs 基结构可以作为“ whispering gallery mode”（WGM）谐振器，在其中产生准 whispering gallery 模式（quasi-WGM）。这些准 whispering gallery 模式在可见和红外范围的光致发光光谱中被实验观察到。为了设计免疫传感系统，将抗 GVA 抗体固定在硅/ZnO-NRs 的表面上，从而形成硅/ZnO-NRs/抗 GVA 结构。抗 GVA 抗体的固定以及硅/ZnO-NRs/抗 GVA 结构与 GVA 蛋白（GVA 抗原）的相互作用导致在可见范围内观察到的光致发光光谱中 WGM 峰的反向移动，这是 ZnO-NRs 的缺陷相关光致发光发射。这里设计的硅/ZnO-NRs/抗 GVA 免疫传感结构在 1-200ng/ml 的浓度范围内表现出对 GVA 抗原的敏感性。讨论了基于硅/ZnO-NRs 的结构在 WGM 注册模式下的生物分析适用性。
