Institute of Biophysics and Cell Engineering of National Academy of Sciences of Belarus, Akademicheskaya St. 27, Minsk 220072, Belarus.
Institute of Atomic Physics and Spectroscopy, University of Latvia, 19, Raina Blvd, 1586 Riga, Latvia.
Molecules. 2020 Jul 10;25(14):3168. doi: 10.3390/molecules25143168.
The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. The novel biosensing platforms based on glass/ZnO NRs/anti-CD5 were tested towards the human T-lymphoblast cell line MOLT-4 derived from patients with acute lymphoblastic leukemia. The control tests towards MOLT-4 cells were performed by using the glass/ZnO NRs/anti-IgG2a system as a negative control. It was shown that the photoluminescence signal of the glass/ZnO NRs/anti-CD5 system increased after adsorption of T-lymphoblast MOLT-4 cells on the biosensor surface. The increase in the ZnO NRs photoluminescence intensity correlated with the number of CD5-positive MOLT-4 cells in the investigated population (controlled by using flow cytometry). Perspectives of the developed ZnO platforms as an efficient cancer cell biosensor were discussed.
目前，精确检测癌细胞仍然是一个全球性的挑战。一维（1D）半导体纳米结构（例如 ZnO 纳米棒）由于在癌症生物传感器中的潜在应用而受到关注。在当前的研究中，证明了使用氧化锌纳米棒（ZnO NRs）平台进行人白血病 T 细胞的光致发光检测的可能性。针对病理细胞表面上的分化簇（CD）蛋白的单克隆抗体（MAB）抗 CD5 已被用作 ZnO 表面上的生物选择性层。选择了抗 CD5 蛋白的最佳浓度以在 ZnO NRs 表面上形成有效的生物选择性层。基于玻璃/ZnO NRs/抗 CD5 的新型生物传感平台针对源自急性淋巴细胞白血病患者的人 T 淋巴母细胞系 MOLT-4 进行了测试。使用玻璃/ZnO NRs/抗 IgG2a 系统作为阴性对照，对 MOLT-4 细胞进行了对照测试。结果表明，在 T 淋巴母细胞 MOLT-4 细胞吸附到生物传感器表面后，玻璃/ZnO NRs/抗 CD5 系统的光致发光信号增加。ZnO NRs 光致发光强度的增加与所研究群体中 CD5 阳性 MOLT-4 细胞的数量相关（通过使用流式细胞术进行控制）。讨论了开发的 ZnO 平台作为有效癌细胞生物传感器的前景。
