Pang Qianqian, Chen Xueqin, Lv Jinhua, Li Teng, Fang Jinggui, Jia Haifeng
Key Laboratory of Genetics and Fruit Development, Horticultural College, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2020 Apr 10;9(4):488. doi: 10.3390/plants9040488.
Triacontanol (TA) is a non-toxic, pollution-free, low-cost, high-efficiency, broad-spectrum plant growth regulator that plays an important role in plant growth and development, but its regulation mechanism of strawberry (Sweet charlie, × Duch.) fruit development is still unclear. In this study, we showed that TA treatment (50 μM) could promote fruit development by up-regulating factors related to fruit ripening-related growth and development. TA increased fruit sugar content and anthocyanin accumulation, and many stress-related enzyme activities. In the meantime, Illumina RNA-Seq technology was used to evaluate the effect of TA treatment on strawberry fruit senescence. The results showed that 9338 differentially expressed genes (DEGs) were obtained, including 4520 up-regulated DEGs and 4818 down-regulated DEGs. We performed gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these DEGs. The results showed that TA treatment caused changes in transcript levels related to cellular processes, hormones and secondary metabolism, such as DNA metabolic processes, flavonoid synthesis, and plant hormone signal transduction. Bioinformatics analysis showed that many transcription factors were related to fruit maturity. Taken together, this study will provide new insights into the mechanism of strawberry development and postharvest response to TA treatment.
三十烷醇（TA）是一种无毒、无污染、低成本、高效、广谱的植物生长调节剂，在植物生长发育中起着重要作用，但其对草莓（甜查理，×杜钦）果实发育的调控机制仍不清楚。在本研究中，我们发现TA处理（50μM）可通过上调与果实成熟相关的生长发育因子来促进果实发育。TA增加了果实糖分含量和花青素积累，并提高了许多与胁迫相关的酶活性。同时，利用Illumina RNA-Seq技术评估TA处理对草莓果实衰老的影响。结果表明，共获得9338个差异表达基因（DEG），其中上调的DEG有4520个，下调的DEG有4818个。我们对这些DEG进行了基因本体论（GO）富集和京都基因与基因组百科全书（KEGG）分析。结果表明，TA处理导致了与细胞过程、激素和次生代谢相关的转录水平变化，如DNA代谢过程、类黄酮合成和植物激素信号转导。生物信息学分析表明，许多转录因子与果实成熟有关。综上所述，本研究将为草莓发育机制以及采后对TA处理的响应提供新的见解。
