Li Shuangtao, Chang Linlin, Sun Rui, Dong Jing, Zhong Chuanfei, Gao Yongshun, Zhang Hongli, Wei Lingzhi, Wei Yongqing, Zhang Yuntao, Wang Guixia, Sun Jian
Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Beijing Engineering Research Center for Strawberry, Beijing, China.
Front Plant Sci. 2022 Sep 6;13:996765. doi: 10.3389/fpls.2022.996765. eCollection 2022.
Strawberry ( × Duch) are sensitive to salt stress, and breeding salt-tolerant strawberry cultivars is the primary method to develop resistance to increased soil salinization. However, the underlying molecular mechanisms mediating the response of strawberry to salinity stress remain largely unknown. This study evaluated the salinity tolerance of 24 strawberry varieties, and transcriptomic and metabolomic analysis were performed of 'Sweet Charlie' (salt-tolerant) and 'Benihoppe' (salt-sensitive) to explore salt tolerance mechanisms in strawberry. Compared with the control, we identified 3412 differentially expressed genes (DEGs) and 209 differentially accumulated metabolites (DAMs) in 'Benihoppe,' and 5102 DEGs and 230 DAMs in 'Sweet Charlie.' DEGs Gene Ontology (GO) enrichment analyses indicated that the DEGs in 'Benihoppe' were enriched for ion homeostasis related terms, while in 'Sweet Charlie,' terms related to cell wall remodeling were over-represented. DEGs related to ion homeostasis and cell wall remodeling exhibited differential expression patterns in 'Benihoppe' and 'Sweet Charlie.' In 'Benihoppe,' 21 ion homeostasis-related DEGs and 32 cell wall remodeling-related DEGs were upregulated, while 23 ion homeostasis-related DEGs and 138 cell wall remodeling-related DEGs were downregulated. In 'Sweet Charlie,' 72 ion homeostasis-related DEGs and 275 cell wall remodeling-related DEGs were upregulated, while 11 ion homeostasis-related DEGs and 20 cell wall remodeling-related DEGs were downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed only four KEGG enriched pathways were shared between 'Benihoppe' and 'Sweet Charlie,' including flavonoid biosynthesis, phenylalanine metabolism, phenylpropanoid biosynthesis and ubiquinone, and other terpenoid-quinone biosynthesis. Integrating the results of transcriptomic and metabolomics analyses showed that adenosine triphosphate-binding cassette (ABC) transporters and flavonoid pathway genes might play important roles in the salt stress response in strawberry, and DAMs and DEGs related to ABC transporter and flavonoid pathways were differentially expressed or accumulated. The results of this study reveal that cell wall remodeling and ABC transporters contribute to the response to salt stress in strawberry, and that related genes showed differential expression patterns in varieties with different salt tolerances. These findings provide new insights into the underlying molecular mechanism of strawberry response to salt stress and suggest potential targets for the breeding of salt-tolerant strawberry varieties.
草莓（× 杜氏）对盐胁迫敏感，培育耐盐草莓品种是增强对土壤盐渍化加剧抗性的主要方法。然而，介导草莓对盐胁迫响应的潜在分子机制仍 largely 未知。本研究评估了 24 个草莓品种的耐盐性，并对‘甜查理’（耐盐）和‘红颜’（盐敏感）进行了转录组和代谢组分析，以探索草莓的耐盐机制。与对照相比，我们在‘红颜’中鉴定出 3412 个差异表达基因（DEGs）和 209 个差异积累代谢物（DAMs），在‘甜查理’中鉴定出 5102 个 DEGs 和 230 个 DAMs。DEGs 的基因本体（GO）富集分析表明，‘红颜’中的 DEGs 富集于与离子稳态相关的术语，而在‘甜查理’中，与细胞壁重塑相关的术语过度表达。与离子稳态和细胞壁重塑相关的 DEGs 在‘红颜’和‘甜查理’中表现出不同的表达模式。在‘红颜’中，21 个与离子稳态相关的 DEGs 和 32 个与细胞壁重塑相关的 DEGs 上调，而 23 个与离子稳态相关的 DEGs 和 138 个与细胞壁重塑相关的 DEGs 下调。在‘甜查理’中，72 个与离子稳态相关的 DEGs 和 275 个与细胞壁重塑相关的 DEGs 上调，而 11 个与离子稳态相关的 DEGs 和 20 个与细胞壁重塑相关的 DEGs 下调。京都基因与基因组百科全书（KEGG）通路分析表明，‘红颜’和‘甜查理’之间仅共享四条 KEGG 富集通路，包括类黄酮生物合成、苯丙氨酸代谢、苯丙烷类生物合成以及泛醌和其他萜类 - 醌生物合成。整合转录组和代谢组分析结果表明，三磷酸腺苷结合盒（ABC）转运蛋白和类黄酮途径基因可能在草莓对盐胁迫的响应中起重要作用，与 ABC 转运蛋白和类黄酮途径相关的 DAMs 和 DEGs 存在差异表达或积累。本研究结果表明，细胞壁重塑和 ABC 转运蛋白有助于草莓对盐胁迫的响应，并且相关基因在不同耐盐性品种中表现出不同的表达模式。这些发现为草莓对盐胁迫响应的潜在分子机制提供了新的见解，并为耐盐草莓品种的育种提出了潜在靶点。
