Nguyen Nhu-Hoa, Vu Huyen-Trang, Le Ngoc-Diep, Nguyen Thanh-Diem, Duong Hoa-Xo, Tran Hoang-Dung
Faculty of Biology, Ho Chi Minh City University of Education, 280 An Duong Vuong Street, Ward 4, District 5, Ho Chi Minh 72711, Vietnam.
Faculty of Biotechnology, Nguyen Tat Thanh University, 298A-300A Nguyen Tat Thanh Street, Ward 13, District 4, Ho Chi Minh 72820, Vietnam.
Biology (Basel). 2020 Apr 10;9(4):76. doi: 10.3390/biology9040076.
has been widely used not only as ornamental plants but also as food and medicines. The identification and evaluation of the genetic diversity of species support the conservation of genetic resources of endemic species. Uniquely identifying species used as medicines helps avoid misuse of medicinal herbs. However, it is challenging to identify species morphologically during their immature stage. Based on the DNA barcoding method, it is now possible to efficiently identify species in a shorter time. In this study, the genetic diversity of 76 samples from Southern Vietnam was investigated based on the ITS (Internal transcribed spacer), ITS2, K (Maturase_K), L (ribulose-bisphosphate carboxylase large subunit) and HA (the internal space of the gene coding histidine transfer RNA (trnH) and gene coding protein D1, a polypeptide of the photosystem I reaction center (psaB)) regions. The ITS region was found to have the best identification potential. Nineteen out of 24 species were identified based on phylogenetic tree and Indel information of this region. Among these, seven identified species were used as medicinal herbs. The results of this research contributed to the conservation, propagation, and hybridization of indigenous species in Southern Vietnam.
它不仅作为观赏植物被广泛使用，还被用作食物和药物。对物种遗传多样性的鉴定和评估有助于保护地方特有物种的遗传资源。准确鉴定用作药物的物种有助于避免草药的误用。然而，在其未成熟阶段通过形态学鉴定物种具有挑战性。基于DNA条形码方法，现在有可能在更短的时间内高效鉴定物种。在本研究中，基于ITS（内转录间隔区）、ITS2、K（成熟酶K）、L（核酮糖-1,5-二磷酸羧化酶大亚基）和HA（编码组氨酸转运RNA（trnH）的基因内部间隔区以及编码光系统I反应中心多肽D1（psaB）的基因）区域，对来自越南南部的76个样本的遗传多样性进行了研究。发现ITS区域具有最佳的鉴定潜力。基于该区域的系统发育树和插入缺失信息，24个物种中的19个被鉴定出来。其中，7个已鉴定的物种被用作草药。本研究结果有助于越南南部本土物种的保护、繁殖和杂交。
