State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P.R. China.
Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
Anal Chem. 2020 May 5;92(9):6555-6563. doi: 10.1021/acs.analchem.0c00197. Epub 2020 Apr 27.
The orderly organelle interaction network is essential for normal biological activity of cells. However, the mechanism of orderly organelle interaction remains elusive. In this report, we analyzed the structure characteristics of the cell membrane, endocytic vesicles, and the Golgi membrane through a high-resolution imaging technique and further comprehensively investigated the vesicle-transport process via epidermal growth factor receptor endocytosis and a recycling pathway using a real-time fluorescence tracing method. Our data suggest that orderly vesicle transport is due to protein protrusion from the outer surface of endocytic vesicles and that full membrane fusion between homotypic endocytic vesicles is a result of the rough outer surface. Finally, the kiss-and-run method, which is utilized by endocytic vesicles to communicate with the -Golgi network (TGN) is attributed to a dense protein layer at the outer surface of the TGN. In summary, by combining static structural analysis with dynamic tracing, we elucidate the mechanism of orderly vesicle transport from the overall structural features of the membrane. This work provides insight into the structural mechanisms underlying vital biological processes involving organelle interactions at the molecular level.
有序的细胞器相互作用网络对于细胞的正常生物活性至关重要。然而，有序细胞器相互作用的机制仍难以捉摸。在本报告中，我们通过高分辨率成像技术分析了细胞膜、内吞小泡和高尔基体膜的结构特征，进一步通过实时荧光追踪法全面研究了表皮生长因子受体内吞和再循环途径中的囊泡运输过程。我们的数据表明，有序的囊泡运输是由于内吞小泡外表面的蛋白质突起引起的，而同源内吞小泡之间的完全膜融合是由于粗糙的外表面所致。最后，内吞小泡与 -高尔基体网络（TGN）之间的“亲吻-跑开”方法归因于 TGN 外表面的致密蛋白质层。总之，通过将静态结构分析与动态追踪相结合，我们从膜的整体结构特征阐明了囊泡运输的机制。这项工作为涉及细胞器相互作用的重要生物学过程提供了分子水平上的结构机制见解。
