Cheng Yuqi, Cong Lili, Qu Xiaozhang, Zhao Junyi, Chen Jiamin, Li Ping, Shi Wei, Xu Weiqing, Xu Shuping
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, People's Republic of China.
Institute of Theoretical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, People's Republic of China.
Mikrochim Acta. 2023 Mar 9;190(4):124. doi: 10.1007/s00604-023-05705-2.
A surface-enhanced Raman scattering (SERS)/fluorescence dual-mode nanoprobe was proposed to assess anti-diabetic drug actions from the expression level of the epidermal growth factor receptor (EGFR), which is a significant biomarker of breast cancers. The nanoprobe has a raspberry shape, prepared by coating a dye-doped silica nanosphere with a mass of SERS tags, which gives high gains in fluorescence imaging and SERS measurement. The in situ detection of EGFR on the cell membrane surfaces after drug actions was achieved by using this nanoprobe, and the detection results agree with the enzyme-linked immunosorbent assay (ELISA) kit. Our study suggests that rosiglitazone hydrochloride (RH) may be a potential drug for diabetic patients with breast cancer, while the anti-cancer effect of metformin hydrochloride (MH) is debatable since MH slightly promotes the EGFR expression of MCF-7 cells in this study. This sensing platform endows more feasibility for highly sensitive and accurate feedback of pesticide effects at the membrane protein level.
提出了一种表面增强拉曼散射（SERS）/荧光双模纳米探针，用于从表皮生长因子受体（EGFR）的表达水平评估抗糖尿病药物的作用，EGFR是乳腺癌的一个重要生物标志物。该纳米探针呈覆盆子形状，通过用大量SERS标签包裹染料掺杂的二氧化硅纳米球制备而成，在荧光成像和SERS测量中具有高增益。使用该纳米探针实现了药物作用后细胞膜表面EGFR的原位检测，检测结果与酶联免疫吸附测定（ELISA）试剂盒一致。我们的研究表明，盐酸罗格列酮（RH）可能是患有乳腺癌的糖尿病患者的一种潜在药物，而盐酸二甲双胍（MH）的抗癌作用存在争议，因为在本研究中MH略微促进了MCF-7细胞的EGFR表达。该传感平台为在膜蛋白水平上高灵敏度和准确反馈农药效应赋予了更多可行性。
