Violante Ivana M P, Carollo Carlos A, Silva Larissa I, Oliveira Adna Q C, Pardinho Flavia C, Garcez Walmir Silva, Garcez Fernanda R, Oliveira Ruberlei Godinho de, Arunachalam Karuppusamy, de Oliveira Martins Domingos Tabajara
Area of Pharmacology, Department of Basic Sciences in Health, Faculty of Medicine, Federal University of Mato Grosso (UFMT), Cuiabá, Mato Grosso, Brazil.
Pharmacognosy Laboratory, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Nat Prod Res. 2021 Dec;35(23):5287-5293. doi: 10.1080/14786419.2020.1753050. Epub 2020 Apr 14.
The hydroethanolic extract obtained from the dry leaves of (HEFc) underwent several fractionations by different chromatographic techniques. The ethyl acetate and dichloromethane fraction were subjected to phytochemical analysis, resulting in the identification and isolation of scutellarein () and in a fraction rich in carajurone (). They were tested for cytotoxicity in CHO-K1 and the antibacterial activity and mode of action by assays. The HEFc and scutellarein () presented no cytotoxicity. The results showed good antibacterial effect of HEFc against and and moderate activity for , and . The fraction containing the compound carajurone () showed good activity against and and moderate activity against . Scutellarein () showed no activity against the bacteria tested. HEFc antibacterial mode of action appeared to be associated with changes in the permeability of bacterial membranes and nucleotide leakage.
从[植物名称]干燥叶片中获得的乙醇提取物（HEFc）通过不同的色谱技术进行了多次分离。乙酸乙酯和二氯甲烷馏分进行了植物化学分析，结果鉴定并分离出了野黄芩素（[具体成分名称]）以及富含卡拉朱龙（[具体成分名称]）的馏分。通过[具体检测方法]对它们在CHO - K1细胞中的细胞毒性、抗菌活性及作用方式进行了测试。HEFc和野黄芩素（[具体成分名称]）未表现出细胞毒性。结果表明，HEFc对[具体细菌名称1]和[具体细菌名称2]具有良好的抗菌效果，对[具体细菌名称3]、[具体细菌名称4]和[具体细菌名称5]具有中等活性。含有化合物卡拉朱龙（[具体成分名称]）的馏分对[具体细菌名称1]和[具体细菌名称2]表现出良好活性，对[具体细菌名称3]具有中等活性。野黄芩素（[具体成分名称]）对所测试的细菌无活性。HEFc的抗菌作用方式似乎与细菌膜通透性变化和核苷酸泄漏有关。
