National Institute of Plant Genome Research , New Delhi, India.
Plant Signal Behav. 2020 Jun 2;15(6):1754621. doi: 10.1080/15592324.2020.1754621. Epub 2020 Apr 14.
In our recent publication, we have shown that a member of the Laccase family, ( acted as a negative regulator of lignin deposition in the root xylem tissue of messenger RNA (mRNA) level was post-transcriptionally regulated by microRNA 397b, which showed increased expression under water and phosphate deficiency, resulting in the downregulation of LAC2 expression In this report, we have investigated root growth and lignin deposition in an economically important legume crop chickpea ( L.) in response to natural drought in soil-grown condition. In contrast to the growth retardation of root in mannitol-supplemented medium, chickpea root showed an increase in length in low soil moisture condition. Lignin estimation in the primary root showed an increase in lignin content, which was substantiated by staining of root xylem. Drought treatment enhanced the expression of four out of six genes tested, while the expression of two was downregulated. Our preliminary study indicateed a molecular mechanism of lignin deposition in chickpea root xylem during drought.
在我们最近的研究中，我们表明漆酶家族的一个成员（在木质部组织中起木质素沉积的负调控因子的作用），在信使 RNA（mRNA）水平上受 microRNA 397b 的转录后调控，microRNA 397b 在水分和磷缺乏的情况下表达增加，导致 LAC2 表达下调。在本报告中，我们研究了经济上重要的豆科作物鹰嘴豆（L.）在土壤生长条件下对自然干旱的反应中的根生长和木质素沉积。与甘露醇补充培养基中 的生长抑制相反，鹰嘴豆根在低土壤水分条件下表现出长度的增加。在主根中木质素的测定显示木质素含量增加，根木质部的染色证实了这一点。干旱处理增强了测试的六个基因中的四个的表达，而两个基因的表达被下调。我们的初步研究表明了鹰嘴豆根木质部在干旱期间木质素沉积的分子机制。
