Zhao Hui, Gao Yanrong, Song Tongqiu, Jia Yuqiao
School of Public Health, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014060, China.
Wei Sheng Yan Jiu. 2020 Mar;49(2):242-248. doi: 10.19813/j.cnki.weishengyanjiu.2020.02.013.
To understand the exposure levels of polycyclic aromatic hydrocarbons(PAHs) and the expression of interleukin-2(IL-2), interferon-γ(IFN-γ), interleukin-4(IL-4), interleukin-10(IL-10) in peripheral blood of coke oven workers exposed to coke oven emissions(COEs). The other purpose of this study was to understand the performance of IFN-γ and IL-10 epigenetic mechanisms in COEs exposure damage.
The 85 workers exposed to COEs in a coking plant were randomly selected as the exposure group. The 47 workers who were exposed to non-COEs in the coking plant were used as the control group. The morning urine of the exposure group and the control group were subjected to detection of 1-OHPyr levels with alkaline hydrolysis High-performance liquid chromatography fluorescence, urine creatinine correction. The peripheral venous blood were subjected to detection of the expression of IL-2, IFN-γ, IL-4 and IL-10 with enzyme-linked immunosorbent assay. And methylation levels of IFN-γ and IL-10 were analyzed by time of flight mass spectrometry.
The urine 1-hyroxy-pyrene(1-OHPyr) content of coke oven workers was higher than that of the control group(F=12. 446, P<0. 05). The urine 1-OHPyr content of the furnace side and the furnace top were higher than the control group, and the differences were statistically significant. Compared with the control group, serum IL-2 content of coke oven workers decreased(F=14. 774, P<0. 05), and serum IFN-γ content of coke oven workers decreased(F=46. 379, P<0. 05), the serum IL-4 content of coke oven workers increased(F=17. 426, P<0. 05), the serum IL-10 content of coke oven workers increased(F=33. 515, P<0. 05), and the TH1/TH2 ratio of coke oven workers decreased(F=21. 677, P<0. 05). In the exposed group, the level of IFN-γ in the top of the furnace was higher than that in the bottom of the furnace. The difference was statistically significant. The level of IL-10 in the top and bottom of the furnace was lower than that in the furnace. The difference was statistically significant. The IL-10 CpG-11, CpG-15 and mean methylation rates in the exposed group were lower than those in the control group, and the differences were statistically significant. The methylation rate of IFN-γ CpG-5 in the exposed group was higher than that in the control group, and the difference was statistically significant. The urine 1-OHPyr content of coke oven workers was negatively correlated with TH1/TH2 ratio and IFN-γ expression level, and positively correlated with IL-4 and IL-10 levels. The IL-10 CpG-11, CpG-15 methylation rate decreased with increasing urine 1-OHPyr concentration.
The side and top of the furnace worker exposed to COEs were the key targets for occupational health. The exposure of coke oven workers to COEs affected the expression of immunoregulatory cytokines. The exposure of COEs caused the change of IL-10 methylation rate.
了解焦炉逸散物（COEs）暴露对焦炉工人外周血中多环芳烃（PAHs）暴露水平及白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）、白细胞介素-10（IL-10）表达的影响。本研究的另一个目的是了解IFN-γ和IL-10表观遗传机制在COEs暴露损伤中的表现。
随机选取某焦化厂85名COEs暴露工人作为暴露组。选取该厂47名未暴露于COEs的工人作为对照组。采用碱性水解高效液相色谱荧光法检测暴露组和对照组晨尿中1-羟基芘（1-OHPyr）水平，并进行尿肌酐校正。采用酶联免疫吸附测定法检测外周静脉血中IL-2、IFN-γ、IL-4和IL-10的表达。采用飞行时间质谱法分析IFN-γ和IL-10的甲基化水平。
焦炉工人尿中1-羟基芘（1-OHPyr）含量高于对照组（F=12.446，P<0.05）。炉侧和炉顶工人尿中1-OHPyr含量高于对照组，差异有统计学意义。与对照组相比，焦炉工人血清IL-2含量降低（F=14.774，P<0.05），血清IFN-γ含量降低（F=46.379，P<0.05），血清IL-4含量升高（F=17.426，P<0.05），血清IL-10含量升高（F=33.515，P<0.05），焦炉工人TH1/TH2比值降低（F=21.677，P<0.05）。暴露组中，炉顶IFN-γ水平高于炉底，差异有统计学意义。炉顶和炉底IL-10水平低于炉中，差异有统计学意义。暴露组IL-10 CpG-11、CpG-
