Sundaresha S, Sharma Sanjeev, Shandil Rajesh K, Sharma Sadhana, Thakur Vandana, Bhardwaj Vinay, Kaushik Surinder K, Singh Bir Pal, Chakrabarti Swarup K
ICAR-Central Potato Research Institute, Shimla - 171 001, Himachal Pradesh, India.
ICAR-National Bureau of Plant Genetic Resources, New Delhi -110012, India.
Funct Plant Biol. 2018 Oct;45(10):1026-1037. doi: 10.1071/FP17299.
Earlier studies have shown that level of late blight resistance conferred by the classical R gene (RB Rpi-blb1) is dependent on genetic background of the recipient genotype. This was revealed in the analysis of late blight response that belonged to a group of F1 progeny obtained from the cross between Kufri Jyoti and SP951, which showed wide variation in late blight resistance response in spite of possessing the same RB gene. The global gene expression pattern in the RB potato lines was studied in response to late blight infection using cDNA microarray analysis to reveal the background effect. Leaf samples were collected at 0, 24, 72 and 120h post inoculation (hpi) with Phytophthora infestans for gene expression analysis using 61031 gene sequences. Significantly upregulated (1477) and downregulated (4245) genes common in the RB-transgenic F1 lines at 24 and 72 hpi were classified into several categories based on GO identifiers and majority of genes were assigned putative biological functions. Highest expression of an NBS-LRR along with protease, pectin esterase inhibitors, chaperones and reactive oxygen species genes were observed which affirmed a significant role of these categories in the defence response of RB-KJ lines. Results suggest that the immune priming of plant receptors are likely to be involved in stability and functionality of RB to induce resistance against P. infestans. This study is important for effective deployment of RB gene in the host background and contributes immensely to scientific understanding of R gene interaction with host protein complexes to regulate defence system in plants.
早期研究表明，经典R基因（RB Rpi-blb1）赋予的晚疫病抗性水平取决于受体基因型的遗传背景。这在对晚疫病反应的分析中得以揭示，该反应属于从Kufri Jyoti和SP951杂交获得的一组F1后代，尽管拥有相同的RB基因，但它们在晚疫病抗性反应中表现出广泛差异。利用cDNA微阵列分析研究了RB马铃薯品系对晚疫病感染的全局基因表达模式，以揭示背景效应。在接种致病疫霉后0、24、72和120小时（hpi）采集叶片样本，使用61031个基因序列进行基因表达分析。在24和72 hpi时，RB转基因F1品系中显著上调（1477个）和下调（4245个）的共同基因根据基因本体标识符被分类为几个类别，并且大多数基因被赋予了推定的生物学功能。观察到一个NBS-LRR以及蛋白酶、果胶酯酶抑制剂、伴侣蛋白和活性氧物种基因的最高表达，这证实了这些类别在RB-KJ品系防御反应中的重要作用。结果表明，植物受体的免疫引发可能参与RB诱导对致病疫霉抗性的稳定性和功能性。这项研究对于在宿主背景中有效部署RB基因很重要，并极大地有助于科学理解R基因与宿主蛋白复合物相互作用以调节植物防御系统。
