Guangxi Key Laboratory of Sugarcane Biology, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, China.
College of Agriculture, Guangxi University, Nanning 530004, China.
Int J Mol Sci. 2024 Aug 22;25(16):9129. doi: 10.3390/ijms25169129.
Sugarcane smut, caused by the fungus (Sydow), significantly affects sugarcane crops worldwide. Infected plants develop whip-like structures known as sori. Significant variations in these whip lengths are commonly observed, but the physiological and molecular differences causing these morphological differences remain poorly documented. To address this, we employed conventional microbe isolation, metagenomic, and metabolomic techniques to investigate smut-infected sugarcane stems and whips of varying lengths. Metagenomics analysis revealed a diverse fungal community in the sugarcane whips, with and genera notably present (>1%) in long whips. Isolation techniques confirmed these findings. Ultra-performance liquid chromatography analysis (UHPLC-MS/MS) showed high levels of gibberellin hormones (GA, GA, GA, GA, and GA) in long whips, with GA and GA found exclusively in long whips and stems. Among the prominent genera present within long whips, was solely positively correlated with these gibberellin (GA) hormones, with the exception of GA, which was positively correlated with . KEGG enrichment analysis linked these hormones to pathways like diterpenoid biosynthesis and plant hormone signal transduction. These findings suggest that may influence GA production leading to whip elongation. Our study reveals fungal dynamics and gibberellin responses in sugarcane smut whips. Future research will explore the related molecular gibberellin synthesis mechanisms.
甘蔗黑粉病由真菌（ Sydow ）引起，对全球的甘蔗作物造成严重影响。受感染的植物会形成鞭状结构，称为孢子囊。这些鞭状结构的长度存在显著差异，但导致这些形态差异的生理和分子差异仍记录甚少。为了解决这个问题，我们采用了常规微生物分离、宏基因组学和代谢组学技术，研究了不同长度的受感染甘蔗茎和鞭状结构。宏基因组学分析显示，甘蔗鞭状结构中存在多样化的真菌群落， 和 属（>1%）在长鞭状结构中显著存在。分离技术证实了这些发现。超高效液相色谱分析（UHPLC-MS/MS）显示，长鞭状结构中存在高水平的赤霉素激素（GA、GA、GA、GA 和 GA），GA 和 GA 仅存在于长鞭状结构和茎中。在长鞭状结构中存在的主要属中， 与这些赤霉素（GA）激素呈正相关，除了 GA，它与 呈正相关。KEGG 富集分析将这些激素与二萜生物合成和植物激素信号转导等途径联系起来。这些发现表明， 可能影响 GA 的产生，导致鞭状结构伸长。我们的研究揭示了甘蔗黑粉病鞭状结构中的真菌动态和赤霉素响应。未来的研究将探索相关的分子赤霉素合成机制。
