Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan.
Division of Endocrinology and Metabolism, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan, 33305, Taiwan.
Adv Biosyst. 2020 Mar;4(3):e1900254. doi: 10.1002/adbi.201900254. Epub 2020 Feb 10.
Islet transplantation has been demonstrated to be a promising therapy for type 1 diabetes mellitus. Although it is a minimally invasive operating procedure and provides easy access for graft monitoring, subcutaneous transplantation of the islet only has limited therapeutic outcomes, owing to the poor capacity of skin tissue to foster revascularization in a short period. Herein, 3D cell spheroids of clinically accessible umbilical cord blood mesenchymal stem cells and human umbilical vein endothelial cells are formed and employed for codelivery with β cells subcutaneously. The 3D stem cell spheroids, which can secrete multiple proangiogenic and prosurvival growth factors, induce robust angiogenesis and prevent β cell graft death, as indicated by the results of in vivo bioluminescent tracking and histological analysis. These experimental data highlight the efficacy of the 3D stem cell spheroids that are fabricated using translationally applicable cell types in promoting the survival and function of subcutaneously transplanted β cells.
胰岛细胞移植已被证明是治疗 1 型糖尿病的一种很有前途的疗法。尽管它是一种微创操作程序，并且便于进行移植物监测，但由于皮肤组织在短时间内促进再血管化的能力有限，因此胰岛的皮下移植仅具有有限的治疗效果。在此，形成了临床上可获得的脐带血间充质干细胞和人脐静脉内皮细胞的 3D 细胞球体，并将其与β细胞一起用于皮下共递送。3D 干细胞球体可分泌多种促血管生成和促生存生长因子，可诱导强有力的血管生成并防止β细胞移植物死亡，这一点通过体内生物发光追踪和组织学分析得到了证实。这些实验数据突出了使用可转化应用的细胞类型制造的 3D 干细胞球体在促进皮下移植的β细胞的存活和功能方面的功效。
