Department of Pharmacy, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 3 East Qingchun Road, Hangzhou, 310016, China.
Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310006, People's Republic of China.
BMC Complement Med Ther. 2020 Mar 23;20(1):95. doi: 10.1186/s12906-020-02885-9.
To explore the mechanism of action of Tripterygium wilfordii Hook (TWH) in the treatment of Crohn's disease (CD) by network pharmacology.
Traditional Chinese Medicine Systems Pharmacology database and analysis platform (TCMSP) was used to obtain the active constituents and targets of TWH. "Crohn's disease" was used as a search term to search for related targets of CD from GeneCards database and OMIM database, thereby obtaining the targets of TWH against CD. The Cytoscape 3.7.1 software was used to construct a Chinese medicine compound-target network and STRING database to construct a protein-protein interaction network (PPI). The DAVID 6.8 online tool was used to perform gene ontology (GO) and kyoto encyclopedia of genes and genome (KEGG) pathway enrichment analysis of overlapping targets.
The database results showed that there were 30 active ingredients (14 key active ingredients) in TWH and 36 targets were screened out for CD treatment. Network analysis indicated that main targets of main active components of TWH were target genes such as VEGFA, MAPK8 and CASP3, which are involved in the regulation of cancer pathway, TNF signal pathway, hepatitis B pathway, apoptosis pathway, NF-kappa B signal pathway and so forth.
TWH can play a multi-target and multi-channel synergistic treatment of CD by anti-angiogenesis, anti-apoptosis, anti-inflammation and immune regulation.
通过网络药理学探讨雷公藤（TWH）治疗克罗恩病（CD）的作用机制。
利用中药系统药理学数据库和分析平台（TCMSP）获取 TWH 的活性成分和靶点。从 GeneCards 数据库和 OMIM 数据库中以“克罗恩病”为检索词，检索 CD 的相关靶点，从而获得 TWH 治疗 CD 的靶点。使用 Cytoscape 3.7.1 软件构建中药化合物-靶点网络，利用 STRING 数据库构建蛋白质-蛋白质相互作用网络（PPI）。使用 DAVID 6.8 在线工具对重叠靶点进行基因本体（GO）和京都基因与基因组百科全书（KEGG）通路富集分析。
数据库结果显示，TWH 中有 30 种活性成分（14 种关键活性成分），筛选出 36 个治疗 CD 的靶点。网络分析表明，TWH 主要活性成分的主要靶点是 VEGFA、MAPK8 和 CASP3 等基因，这些基因参与癌症途径、TNF 信号通路、乙型肝炎途径、细胞凋亡途径、NF-κB 信号通路等的调控。
TWH 通过抗血管生成、抗细胞凋亡、抗炎和免疫调节，发挥多靶点、多通道协同治疗 CD 的作用。
