保守的昆虫二氧化碳受体亚基对气味检测的贡献。
Contributions of the Conserved Insect Carbon Dioxide Receptor Subunits to Odor Detection.
作者信息
Kumar Arun, Tauxe Genevieve M, Perry Sarah, Scott Christi Ann, Dahanukar Anupama, Ray Anandasankar
机构信息
Department of Molecular, Cell and Systems Biology, University of California Riverside, Riverside, CA 92521, USA.
Department of Entomology, University of California Riverside, Riverside, CA 92521, USA.
出版信息
Cell Rep. 2020 Apr 14;31(2):107510. doi: 10.1016/j.celrep.2020.03.074.
The CO receptor in mosquitoes is broadly tuned to detect many diverse odorants. The receptor consists of three subunits (Gr1, Gr2, and Gr3) in mosquitoes but only two subunits in Drosophila: Gr21a (Gr1 ortholog) and Gr63a (Gr3 ortholog). We demonstrate that Gr21a is required for CO responses in Drosophila, as has been shown for Gr63a. Next, we generate a Drosophila double mutant for Gr21a and Gr63a, and in this background, we functionally express combinations of Aedes Gr1, Gr2, and Gr3 genes in the CO empty neuron. Only two subunits, Gr2 and Gr3, suffice for response to CO. Addition of Gr1 increases sensitivity to CO, whereas it decreases the response to pyridine. The inhibitory effect of the antagonist isobutyric acid is observed upon addition of Gr1. Gr1 therefore increases the diversity of ligands of the receptor and modulates the response of the receptor complex.
蚊子中的CO受体具有广泛的调谐能力，可检测多种不同的气味剂。该受体在蚊子中由三个亚基（Gr1、Gr2和Gr3）组成，而在果蝇中只有两个亚基：Gr21a（Gr1的直系同源物）和Gr63a（Gr3的直系同源物）。我们证明，正如Gr63a所显示的那样，Gr21a是果蝇对CO反应所必需的。接下来，我们生成了Gr21a和Gr63a的果蝇双突变体，在此背景下，我们在CO空神经元中功能性表达埃及伊蚊Gr1、Gr2和Gr3基因的组合。只有两个亚基Gr2和Gr3足以对CO产生反应。添加Gr1会增加对CO的敏感性，而会降低对吡啶的反应。添加Gr1后可观察到拮抗剂异丁酸的抑制作用。因此，Gr1增加了受体配体的多样性并调节了受体复合物的反应。
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