Rajendren G, Dominic C J
Department of Zoology, Banaras Hindu University, Varanasi, India.
Exp Clin Endocrinol. 1988 Sep;92(1):13-9. doi: 10.1055/s-0029-1210775.
The luteal function in newly inseminated female mice following exposure to alien males was investigated. The corpora lutea (CL) of newly inseminated females exhibited high delta 5 3 beta hydroxysteroid dehydrogenase (delta 5 3 beta HSD) activity at 24 hr or 48 hr (pro-oestrus) after the beginning of alien male exposure. By contrast, the enzyme activity in the CL at 72 hr (oestrus) after the beginning of alien male exposure was markedly less as compared with that in the CL of unexposed controls. This suggests that the CL of newly inseminated female mice can synthesise progesterone from its substrate at least up to 48 hr after the beginning of alien male exposure. Administration of prolactin to newly inseminated females beginning at 0 hr of alien male exposure prevented implantation failure; the majority of females showed implanted embryos. Prolactin administration starting at 24 hr after the beginning of alien male exposure was only partially effective, and that starting at 48 hr was totally ineffective in preventing implantation failure in newly inseminated females. The results indicate that the CL of newly inseminated females cease to respond to prolactin within 24 hr of alien male exposure, even though they exhibit the capacity to synthesise progesterone (as evidenced by the presence of delta 5 3 beta HSD activity) for a longer period.
研究了新受精的雌性小鼠在接触陌生雄性后的黄体功能。新受精雌性小鼠的黄体在接触陌生雄性开始后的24小时或48小时（动情前期）表现出高δ5 3β羟类固醇脱氢酶（δ5 3βHSD）活性。相比之下，接触陌生雄性开始后72小时（发情期）黄体中的酶活性与未接触的对照组黄体相比明显降低。这表明新受精的雌性小鼠的黄体至少在接触陌生雄性开始后48小时内能够从其底物合成孕酮。从接触陌生雄性0小时开始对新受精雌性小鼠给予催乳素可防止着床失败；大多数雌性小鼠显示有植入胚胎。从接触陌生雄性开始后24小时开始给予催乳素仅部分有效，而从48小时开始给予催乳素在防止新受精雌性小鼠着床失败方面完全无效。结果表明，新受精雌性小鼠的黄体在接触陌生雄性24小时内就不再对催乳素产生反应，尽管它们在更长时间内表现出合成孕酮的能力（由δ5 3βHSD活性证明）。
