α-萘基异硫氰酸酯诱导的大鼠慢性胆管炎中的生化变化

Biochemical changes in alpha-naphthyl isothiocyanate-induced chronic cholangitis in the rat.

作者信息

Nikolaev V, Kerimova M, Naydenova E, Ivanov E, Dontchev N, Adjarov D

机构信息

Institute of Gastroenterology and Nutrition, Medical Academy, Sofia, Bulgaria.

出版信息

Exp Pathol. 1988;33(4):261-3. doi: 10.1016/s0232-1513(88)80083-5.

DOI:10.1016/s0232-1513(88)80083-5

PMID:3229462

Abstract

Male Wistar rats were treated for 3 weeks with alpha-naphthyl isothiocyanate (ANIT, 5.4 mmol per kg food). Chronic necrotic cholangitis without pronounced transaminasemia and hyperbilirubinemia developed. The activity of 5'-nucleotidase in liver plasma membrane preparations was strongly depressed (3.5 times) while the activities of K+-, Na+- and Mg++-ATPases were not affected. The liver microsomal cytochrome P-450 and cytochrome b5 contents decreased. Elevation of reduced glutathione liver content after challenge with ANIT was recorded. The observed biochemical changes may be important for the pathogenesis of ANIT-induced chronic cholangitis.

摘要

雄性Wistar大鼠用异硫氰酸α-萘酯（ANIT，每千克食物5.4毫摩尔）处理3周。出现了无明显转氨酶血症和高胆红素血症的慢性坏死性胆管炎。肝质膜制剂中5'-核苷酸酶的活性强烈降低（3.5倍），而K + -、Na + -和Mg ++ -ATP酶的活性未受影响。肝微粒体细胞色素P-450和细胞色素b5含量降低。记录到ANIT激发后肝脏还原型谷胱甘肽含量升高。观察到的生化变化可能对ANIT诱导的慢性胆管炎的发病机制很重要。