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具有亚纳摩尔亲和力的 C-反应蛋白和乳酸脱氢酶 5 的修饰 DNA 适体。

Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities.

机构信息

NEC Solution Innovators, Ltd., 1-18-7, Shinkiba, Koto-ku, Tokyo 136-8627, Japan.

Graduate School of Integrated Basic Sciences, Nihon University, 3-25-40 Sakurajosui, Setagaya-ku, Tokyo 156-8550, Japan.

出版信息

Int J Mol Sci. 2020 Apr 13;21(8):2683. doi: 10.3390/ijms21082683.

DOI:10.3390/ijms21082683
PMID:32294882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7215426/
Abstract

Human C-reactive protein (CRP) and lactate dehydrogenase are important markers in clinical laboratory testing-the former is used to detect in vivo inflammation, and the latter is used to detect cell necrosis and tissue destruction. We developed aptamers that bind to human CRP and human lactate dehydrogenase-5 (LDH-5) with high affinities (dissociation constants of 6.2 pM and 235 pM, respectively), applying the systematic evolution of ligands by exponential enrichment (SELEX) method, and by using a modified DNA library containing the following base-appended base modifications: analog adenine derivative at the fifth position of uracil (U), analog guanine derivative at the fifth position of uracil (U), and analog adenine derivative at the seventh position of adenine (A). A potential application of these aptamers as sensor elements includes high-sensitivity target detection in point-of-care testing.

摘要

人 C-反应蛋白（CRP）和乳酸脱氢酶是临床实验室检测中的重要标志物——前者用于检测体内炎症，后者用于检测细胞坏死和组织破坏。我们应用指数富集的配体系统进化（SELEX）方法，开发了与人 CRP 和人乳酸脱氢酶-5（LDH-5）具有高亲和力（解离常数分别为 6.2 pM 和 235 pM）的适体，所使用的改良 DNA 文库包含以下碱基修饰的碱基：尿嘧啶（U）第五位的类似腺嘌呤衍生物、尿嘧啶（U）第五位的类似鸟嘌呤衍生物和腺嘌呤（A）第七位的类似腺嘌呤衍生物。这些适体作为传感器元件的一个潜在应用包括在即时检测中进行高灵敏度目标检测。

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