黑箱之内：SELEX 缘何更优？
Inside the Black Box: What Makes SELEX Better?
机构信息
Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, Moscow 124498, Russia.
出版信息
Molecules. 2019 Oct 7;24(19):3598. doi: 10.3390/molecules24193598.
Abstract
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications. Aptamers are isolated from large nucleic acid combinatorial libraries using an iterative selection process called SELEX (Systematic Evolution of Ligands by EXponential enrichment). Since being implemented 30 years ago, the SELEX protocol has undergone many modifications and improvements, but it remains a laborious, time-consuming, and costly method, and the results are not always successful. Each step in the aptamer selection protocol can influence its results. This review discusses key technical points of the SELEX procedure and their influence on the outcome of aptamer selection.
摘要
适体是能够特异性结合靶标的小寡核苷酸，在分析、诊断和治疗应用方面具有巨大的潜力。适体是通过一种称为 SELEX（指数富集的配体系统进化）的迭代选择过程从大型核酸组合文库中分离出来的。自 30 年前实施以来，SELEX 方案经历了许多修改和改进，但它仍然是一种繁琐、耗时和昂贵的方法，而且结果并不总是成功的。适体选择方案的每一步都可能影响其结果。本文讨论了 SELEX 程序的关键技术要点及其对适体选择结果的影响。
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