羽扇豆奶的蛋白质组学特征受提取技术、种皮和品种的影响。

College of Science, Health, Engineering and Education, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.

Department of Food Science and Biotechnology, Faculty of Agriculture, University of Baghdad, Baghdad 10071, Iraq.

Molecules. 2020 Apr 13;25(8):1782. doi: 10.3390/molecules25081782.

Lupin seeds are rich in proteins and other essential ingredients that can help to improve human health. The protein contents in both whole and split seeds of two lupin cultivars (Mandleup and PBA Jurien) were used to produce the lupin milk using the cheesecloth and centrifuge method. Proteins were extracted from the lupin milk using thiourea/urea solubilization. The proteins were separated by a two-dimensional polyacrylamide gel electrophoresis and then identified with mass spectrometry. A total of 230 protein spots were identified, 60 of which showed differential abundances. The cheesecloth separation showed protein extractability much better than that of the centrifuge method for both the cultivars. The results from this study could offer guidance for future comparative analysis and identification of lupin milk protein and provide effective separation technique to determine specific proteins in the cheese-making process.

羽扇豆种子富含蛋白质和其他必需成分，有助于改善人类健康。使用粗棉布和离心机法，利用两种羽扇豆品种（曼德勒普和 PBA 朱里安）的整粒种子和裂粒种子来生产羽扇豆奶。使用硫脲/尿素溶解法从羽扇豆奶中提取蛋白质。通过二维聚丙烯酰胺凝胶电泳分离蛋白质，然后用质谱法进行鉴定。共鉴定出 230 个蛋白斑点，其中 60 个表现出差异丰度。粗棉布分离法对两种品种的蛋白提取率均明显优于离心法。本研究结果可为今后羽扇豆奶蛋白的比较分析和鉴定提供指导，并为奶酪制作过程中特定蛋白的确定提供有效的分离技术。