School of Plant Biology, Faculty of Natural and Agricultural Sciences and The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.
Food Chem. 2012 Dec 1;135(3):1230-8. doi: 10.1016/j.foodchem.2012.05.081. Epub 2012 May 27.
Lupin is an emerging crop worldwide due to its wide range of health benefits. In this study, a comprehensive proteome analysis was conducted using mature seed of four narrow leafed lupin cultivars, Uniharvest, Yorrel, Tanjil and Coromup, through two-dimensional gel electrophoresis followed by mass spectrometric protein sequencing. Two-dimensional gels recognised about 400 protein spots among the cultivars in the 10-100kDa molecular weight and 5.0-8.5 PI ranges. The results revealed a considerable variation of protein expression patterns with a total of 24 proteins showed differential expression among the cultivars, among which 19 were identified as β-conglutin, and 8 were identified as allergenic proteins. Most of the α, δ and γ-conglutins were showing similar expression among the cultivars. Overall, the differentially expressed proteins especially the cultivar specific proteins would be valuable markers for cultivar identification and for screening parental lines of low allergenicity in breeding process.
羽扇豆是一种在全球范围内新兴的作物，因为它具有广泛的健康益处。在这项研究中，通过二维凝胶电泳结合质谱蛋白质测序，对四种窄叶羽扇豆品种（Uniharvest、Yorrel、Tanjil 和 Coromup）的成熟种子进行了全面的蛋白质组分析。二维凝胶在 10-100kDa 分子量和 5.0-8.5 PI 范围内的品种中识别出约 400 个蛋白质斑点。结果显示，蛋白质表达模式存在相当大的差异，共有 24 种蛋白质在品种间表现出差异表达，其中 19 种被鉴定为β-伴大豆球蛋白，8 种被鉴定为过敏原蛋白。大多数 α、δ 和 γ-伴大豆球蛋白在品种间表现出相似的表达。总的来说，差异表达的蛋白质，特别是品种特异性蛋白质，将是品种鉴定和筛选低过敏性育种过程中亲本系的有价值的标记。
