Qiu Ying, Yin Yanhui, Ruan Zhiqiang, Gao Yu, Bian Chao, Chen Jieming, Wang Xiaoai, Pan Xiaofu, Yang Junxing, Shi Qiong, Jiang Wansheng
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China.
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI, Shenzhen 518083, China.
Animals (Basel). 2020 Apr 14;10(4):681. doi: 10.3390/ani10040681.
A The notorious parasite (Ich) has been recorded worldwide in fish species and causes white spot disease, posing major threats and resulting in severe losses to international fish production. Extensively effective strategies for treating Ich are not available yet, and genetic mechanisms of hosts in response to the parasite are still largely unknown. In this study, we selected Kanglang white minnow (KWM, ) to examine its liver transcriptional changes after Ich infection, as white spot disease is one bottleneck problem in exploring this economically important species. We divided the experimental fishes into three groups (control, early-infected, and late-infected) to examine differentially expressed genes (DEGs). A total of 831 DEGs were identified and classified into 128 significantly enriched GO (Gene Ontology) terms and 71 significantly enriched KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways. Most of these terms or pathways were functionally enriched in immunity, inflammatory response, and apoptosis, such as nucleotide-binding oligomerization domain-like (NOD-like) receptor signaling, tumor necrosis factor (TNF) signaling, interleukin-17 (IL-17) signaling, and apoptosis pathways. We also identified 178 putative antimicrobial peptides (AMPs) and AMP precursors based on our previously reported genome assembly of KWM, and revealed that the expressional patterns varied according to different types. In summary, our work reported the first comprehensive transcriptional changes in KWM in response to the exogenous infection of Ich, which would lay a solid foundation for in-depth studies on disease defense or resistant strains selection in this valuable fish.
A 臭名昭著的寄生虫（白点病病原体）已在全球范围内的鱼类物种中被记录，可引发白点病，对国际渔业生产构成重大威胁并导致严重损失。目前尚无广泛有效的治疗白点病的策略，宿主对该寄生虫的遗传反应机制仍 largely 未知。在本研究中，我们选择康朗白甲鱼（KWM）来检测其在感染白点病病原体后的肝脏转录变化，因为白点病是探索这种具有重要经济价值物种时的一个瓶颈问题。我们将实验鱼分为三组（对照组、早期感染组和晚期感染组）以检测差异表达基因（DEG）。共鉴定出 831 个 DEG，并将其分类到 128 个显著富集的基因本体（GO）术语和 71 条显著富集的京都基因与基因组百科全书（KEGG）通路中。这些术语或通路大多在免疫、炎症反应和细胞凋亡方面功能富集，如核苷酸结合寡聚化结构域样（NOD 样）受体信号传导、肿瘤坏死因子（TNF）信号传导、白细胞介素 -17（IL -17）信号传导和细胞凋亡通路。我们还基于之前报道的 KWM 基因组组装鉴定出 178 种假定的抗菌肽（AMP）及其前体，并揭示其表达模式因类型而异。总之，我们的工作首次报道了 KWM 对外源感染白点病病原体的全面转录变化，这将为深入研究这种珍贵鱼类的疾病防御或抗性品系选择奠定坚实基础。
