Cunha Eva, Freitas Ferdinando Bernardino, Braz Berta São, Silva Jorge Moreira da, Tavares Luís, Veiga Ana Salomé, Oliveira Manuela
CIISA-Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal.
Virbac de Portugal Laboratórios, Lda., Rua do Centro Empresarial, Quinta da Beloura, 2710-693 Sintra, Portugal.
Antibiotics (Basel). 2020 Apr 14;9(4):180. doi: 10.3390/antibiotics9040180.
Periodontal disease (PD) is a highly prevalent inflammatory disease in dogs. This disease is initiated by a polymicrobial biofilm on the teeth surface, whose control includes its prevention and removal. Recently, it was shown that nisin displays antimicrobial activity against canine PD-related bacteria. Moreover, guar gum biogel has shown to be a promising topical delivery system for nisin.
In this study we aimed to evaluate the antimicrobial activity of the nisin-biogel in the presence of canine saliva and after a 24-month storage, at different conditions, using a canine oral enterococci collection. We also studied the nisin-biogel cytotoxicity using a Vero cell line and canine primary intestinal fibroblasts.
The presence of saliva hampers nisin-biogel antimicrobial activity, and higher nisin concentrations were required for an effective activity. A significant reduction ( ≤ 0.05) in inhibitory activity was observed for nisin-biogel solutions stored at 37 °C, over a 24-month period, which was not observed with the other conditions. The nisin-biogel showed no cytotoxicity against the cells tested at concentrations up to 200 µg/mL.
Our results confirmed the potential of the nisin-biogel for canine PD control, supporting the development of an in vivo clinical trial.
牙周病（PD）是犬类中一种高度流行的炎症性疾病。这种疾病由牙齿表面的多种微生物生物膜引发，对其控制包括预防和清除。最近，研究表明乳酸链球菌素对犬类牙周病相关细菌具有抗菌活性。此外，瓜尔胶生物凝胶已被证明是一种有前景的乳酸链球菌素局部递送系统。
在本研究中，我们旨在使用犬口腔肠球菌菌株，评估在不同条件下，乳酸链球菌素生物凝胶在犬唾液存在和储存24个月后的抗菌活性。我们还使用Vero细胞系和犬原代肠成纤维细胞研究了乳酸链球菌素生物凝胶的细胞毒性。
唾液的存在会阻碍乳酸链球菌素生物凝胶的抗菌活性，需要更高的乳酸链球菌素浓度才能产生有效活性。在37℃储存24个月的乳酸链球菌素生物凝胶溶液的抑制活性显著降低（≤0.05），其他条件下未观察到这种情况。乳酸链球菌素生物凝胶在浓度高达200μg/mL时对所测试的细胞无细胞毒性。
我们的结果证实了乳酸链球菌素生物凝胶在控制犬类牙周病方面的潜力，支持开展体内临床试验。
