Raposo Catarina, Serrano Isa, Cunha Eva, Couto Maria Patrícia, Lopes Filipa, Casero María, Tavares Luís, Oliveira Manuela
CIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.
Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisboa, Portugal.
Life (Basel). 2023 Jul 27;13(8):1638. doi: 10.3390/life13081638.
Due to poisoning and decline in the food resources of Eurasian vultures, there has been a rise in the number of Griffon () and Cinereous vultures () needing veterinary care. In captivity, vultures often develop oral and other infectious diseases which can affect their survival and the probability of reintroduction in the wild. Therefore, it is important to characterize relevant microbial species present in the oral cavity of vultures, such as spp. In this work, seven spp. isolates previously obtained from and oral swabs collected at two rehabilitation centers in Portugal were characterized regarding their pathogenic enzymatic profile and antimicrobial activity. Isolates were identified by macro and microscopic observation, and PCR and ITS sequencing. Their antimicrobial activity was determined using a collection of pathogenic bacteria and two yeast species. Results showed that 86% of the isolates produced α-hemolysis, 71% expressed DNase, 57% produce lecithinase and lipase, 29% expressed gelatinase, and 29% were biofilm producers. Four isolates showed inhibitory activity against relevant human and veterinary clinical isolates, including , , , and . In conclusion, accurate management programs should consider the benefits and disadvantages of spp. presence in the oral mucosa.
由于中毒以及欧亚兀鹫食物资源的减少，需要兽医护理的兀鹫（髯鹫和秃鹫）数量有所增加。在圈养环境中，兀鹫常常会患上口腔及其他传染病，这可能会影响它们的生存以及放归野外的可能性。因此，明确兀鹫口腔中存在的相关微生物种类，如 菌属，非常重要。在这项研究中，对之前从葡萄牙两个康复中心采集的髯鹫和秃鹫口腔拭子中获得的7株 菌属分离株，就其致病酶谱和抗菌活性进行了鉴定。通过宏观和微观观察、PCR以及ITS测序对分离株进行鉴定。使用一组病原菌和两种酵母菌种来测定它们的抗菌活性。结果显示，86%的分离株产生α溶血，71%表达DNA酶，57%产生卵磷脂酶和脂肪酶，29%表达明胶酶，29%是生物膜产生菌。4株分离株对相关的人类和兽医临床分离株表现出抑制活性，包括 菌、 菌、 菌和 菌。总之，精确的管理方案应考虑 菌属在口腔黏膜中存在的利弊。
