Kaźmińska Karolina, Hallmann Ewelina, Korzeniewska Aleksandra, Niemirowicz-Szczytt Katarzyna, Bartoszewski Grzegorz
Department of Plant Genetics Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, 02-776 Warsaw, Poland.
Department of Functional and Organic Food, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, 02-787 Warsaw, Poland.
Genes (Basel). 2020 Apr 14;11(4):419. doi: 10.3390/genes11040419.
Duchesne squash and pumpkins are cultivated world-wide. fruits are produced for fresh market and are valuable for food processing. Therefore, fruit characteristics and yield are the traits of high economic importance for breeders. To date, the genetic basis of fruit-associated traits in have been poorly understood. In the present study, we evaluated fruit-associated traits and conducted quantitative trait locus (QTL) analysis using recombinant inbred lines (RILs) derived from a cross of two inbred lines with different fruit morphotypes. Phenotypic data for nine fruit traits (earliness, weight, number per plant, yield per plant, length and diameter, shape index, flesh thickness, sucrose content and dry matter content) were collected for RILs in two open-field experiments. Pairwise analysis of the phenotypic data revealed correlations among the fruit and yield-associated traits. Using a previously developed genetic map, we identified 26 QTLs for eight traits. The QTLs were found in 10 locations on eight chromosomes of . The QTLs were detected across experiments and explained up to 41.4% of the observed phenotypic variations. Major-effect QTLs for multiple fruit-associated traits were clustered on chromosome 4, suggesting that this genomic region has been under selection during diversification and/or domestication of .
杜氏南瓜和西葫芦在全球范围内种植。其果实用于新鲜市场销售，对食品加工也很有价值。因此，果实特性和产量是育种者高度重视的经济性状。迄今为止，人们对其果实相关性状的遗传基础了解甚少。在本研究中，我们评估了果实相关性状，并利用两个具有不同果实形态类型的自交系杂交产生的重组自交系（RIL）进行了数量性状位点（QTL）分析。在两个田间试验中收集了RIL的九个果实性状（早熟性、重量、单株数量、单株产量、长度和直径、形状指数、果肉厚度、蔗糖含量和干物质含量）的表型数据。对表型数据的成对分析揭示了果实和产量相关性状之间的相关性。利用先前构建的遗传图谱，我们鉴定出了八个性状的26个QTL。这些QTL位于该物种八条染色体的10个位置上。这些QTL在不同试验中均被检测到，解释了高达41.4%的观察到的表型变异。多个果实相关性状的主效QTL聚集在4号染色体上，这表明该基因组区域在该物种的多样化和/或驯化过程中受到了选择。
